Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population and Clinical Data
2.2. Specimen Collection
2.3. DNA Extraction
2.4. Illumina High-Throughput Sequencing
2.5. Bioinformatic and Statistical Analysis
3. Results
3.1. Clinical Characteristics of Participants
3.2. Sequencing Characteristics
3.3. Bacterial Community Structure and Composition
3.3.1. α- and β-Diversity
3.3.2. Taxonomic Composition of Bacterial Communities
3.3.3. Bacterial Core Community
3.3.4. Differential Abundance of Bacterial Taxa
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | PCa Group | Non-PCa Group | p-Value | |
---|---|---|---|---|
Subjects | 15 | 15 | nd | |
Age, years: mean (SD) | 68 (9) | 69 (8) | 0.621 | |
PSA, ng/mL: median (range) | 9 (3–28) | 9 (3–32) | 0.640 | |
Prostate volume, g: mean (range) | 37 (56) | 79 (230) | 0.015 | |
Charlson index: n (%) | 1 | 2 (13%) | 2 (13%) | 0.799 |
2 | 4 (27%) | 2 (13%) | ||
3 | 4 (27%) | 5 (33%) | ||
4 | 1 (7%) | 3 (20%) | ||
5 | 2 (13%) | 2 (13%) | ||
6 | 1 (7%) | 1 (7%) | ||
7 | 1 (7%) | 0 | ||
ISUP grade: n (%) | 1 | 3 (20%) | nd | nd |
2 | 6 (40%) | nd | ||
3 | 2 (13%) | nd | ||
4 | 2 (13%) | nd | ||
5 | 2 (13%) | nd |
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Gonçalves, M.F.M.; Pina-Vaz, T.; Fernandes, Â.R.; Miranda, I.M.; Silva, C.M.; Rodrigues, A.G.; Lisboa, C. Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System. Cancers 2023, 15, 1423. https://doi.org/10.3390/cancers15051423
Gonçalves MFM, Pina-Vaz T, Fernandes ÂR, Miranda IM, Silva CM, Rodrigues AG, Lisboa C. Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System. Cancers. 2023; 15(5):1423. https://doi.org/10.3390/cancers15051423
Chicago/Turabian StyleGonçalves, Micael F. M., Teresa Pina-Vaz, Ângela Rita Fernandes, Isabel M. Miranda, Carlos Martins Silva, Acácio Gonçalves Rodrigues, and Carmen Lisboa. 2023. "Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System" Cancers 15, no. 5: 1423. https://doi.org/10.3390/cancers15051423