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Open AccessArticle

Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-small Cell Lung Cancer

1
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland
2
Department of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, Poland
3
Department of Thoracic Surgery, Lower Silesian Centre of Lung Diseases, 53-439 Wroclaw, Poland
4
Division of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, Poland
5
Department of Physiotherapy, Wroclaw University School of Physical Education, 51-612 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(10), 1538; https://doi.org/10.3390/cancers11101538
Received: 30 July 2019 / Revised: 4 September 2019 / Accepted: 10 October 2019 / Published: 11 October 2019
(This article belongs to the Special Issue A Special Issue on Tumor Stroma )
Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied in tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim of the study was to determine the irisin expression in NSCLCs in comparison to the clinicopathological factors and expression of TTF-1, p63 and Ki-67. Material and methods: Tissue microarrays with 729 NSCLC and 140 non-malignant lung tissue (NMLT) were used to perform immunohistochemical reactions. Laser Capture Microdissection (LCM) was used to collect cancer and stromal cells from NSCLCs. FNDC5 expression was tested for LCM samples, 75 NSCLCs and 25 NMLTs with the RT-PCR technique. Western-blot, immunofluorescence reaction and RT-PCR assays were performed on lung cancer cell lines. Results: Irisin expression was observed in NSCLC cancer cells and stromal fibroblasts. In cancer cells, irisin expression was decreased in higher grades (G) of malignancy, tumour size (T) and according to lymph node metastasis. In stromal cells, irisin expression was increased in higher G and advanced T. A shorter overall survival was observed in patients with higher irisin expression in NSCLC stromal cells. Conclusions: Irisin expression in stromal fibroblasts may influence cancer cell proliferation and may be a prognostic factor for survival in NSCLC.
Keywords: irisin; Ki-67; TTF-1; p63; non-small cell lung cancer; cancer-associated fibroblasts; cancer irisin; Ki-67; TTF-1; p63; non-small cell lung cancer; cancer-associated fibroblasts; cancer
MDPI and ACS Style

Nowinska, K.; Jablonska, K.; Pawelczyk, K.; Piotrowska, A.; Partynska, A.; Gomulkiewicz, A.; Ciesielska, U.; Katnik, E.; Grzegrzolka, J.; Glatzel-Plucinska, N.; Ratajczak-Wielgomas, K.; Podhorska-Okolow, M.; Dziegiel, P. Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-small Cell Lung Cancer. Cancers 2019, 11, 1538.

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