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Micromachines
Volume 11
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10.3390/mi11090882
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Article

Single-Cell Elasticity Measurement with an Optically Actuated Microrobot

by
István Grexa
1,2,
Tamás Fekete
1,3,
Judit Molnár
1,
Kinga Molnár
1,4,
Gaszton Vizsnyiczai
1,
Pál Ormos
1 and
Lóránd Kelemen
1,*
1
Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary
2
Doctoral School of Interdisciplinary Medicine, University of Szeged, Korányi fasor 10, 6720 Szeged, Hungary
3
Doctoral School of Multidisciplinary Medicine, Dóm tér 9, Hungary University of Szeged, 6720 Szeged, Hungary
4
Doctoral School of Theoretical Medicine, University of Szeged, Korányi fasor 10, 6720 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(9), 882; https://doi.org/10.3390/mi11090882
Submission received: 27 July 2020 / Revised: 15 September 2020 / Accepted: 20 September 2020 / Published: 22 September 2020
(This article belongs to the Special Issue New Trends and Applications in Femtosecond Laser Micromachining)
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Abstract

A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.
Keywords: cell elasticity; endothelial cells; optical micromanipulation; holographic optical tweezers; two-photon polymerization; image processing cell elasticity; endothelial cells; optical micromanipulation; holographic optical tweezers; two-photon polymerization; image processing
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MDPI and ACS Style

Grexa, I.; Fekete, T.; Molnár, J.; Molnár, K.; Vizsnyiczai, G.; Ormos, P.; Kelemen, L. Single-Cell Elasticity Measurement with an Optically Actuated Microrobot. Micromachines 2020, 11, 882. https://doi.org/10.3390/mi11090882

AMA Style

Grexa I, Fekete T, Molnár J, Molnár K, Vizsnyiczai G, Ormos P, Kelemen L. Single-Cell Elasticity Measurement with an Optically Actuated Microrobot. Micromachines. 2020; 11(9):882. https://doi.org/10.3390/mi11090882

Chicago/Turabian Style

Grexa, István, Tamás Fekete, Judit Molnár, Kinga Molnár, Gaszton Vizsnyiczai, Pál Ormos, and Lóránd Kelemen. 2020. "Single-Cell Elasticity Measurement with an Optically Actuated Microrobot" Micromachines 11, no. 9: 882. https://doi.org/10.3390/mi11090882

APA Style

Grexa, I., Fekete, T., Molnár, J., Molnár, K., Vizsnyiczai, G., Ormos, P., & Kelemen, L. (2020). Single-Cell Elasticity Measurement with an Optically Actuated Microrobot. Micromachines, 11(9), 882. https://doi.org/10.3390/mi11090882

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