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Open AccessReview

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

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Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
2
Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Yokohama 226-8502, Japan
3
PRESTO, Japan Science and Technology Agency (JST), Kawaguchi 332-0012, Japan
4
Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan
5
Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai 980-8578, Japan
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(5), 530; https://doi.org/10.3390/mi11050530
Received: 8 May 2020 / Revised: 19 May 2020 / Accepted: 19 May 2020 / Published: 22 May 2020
(This article belongs to the Special Issue Micro and Nano Devices for Cell Analysis)
Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devices for ECL cell assays is introduced. To conclude, the direction of research in this field and its application prospects are described.
Keywords: electrochemiluminescence (ECL); mammalian cell analysis; electrochemical device; electrochemical microscopy; single-cell analysis; ECL luminophore electrochemiluminescence (ECL); mammalian cell analysis; electrochemical device; electrochemical microscopy; single-cell analysis; ECL luminophore
MDPI and ACS Style

Hiramoto, K.; Villani, E.; Iwama, T.; Komatsu, K.; Inagi, S.; Inoue, K.Y.; Nashimoto, Y.; Ino, K.; Shiku, H. Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis. Micromachines 2020, 11, 530.

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