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Open AccessArticle

Simultaneously Quantifying Both Young’s Modulus and Specific Membrane Capacitance of Bladder Cancer Cells with Different Metastatic Potential

1
School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China
2
Shanghai Institute of Intelligent Science and Technology, Tongji University, Shanghai 200092, China
3
School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 249; https://doi.org/10.3390/mi11030249
Received: 1 February 2020 / Revised: 23 February 2020 / Accepted: 25 February 2020 / Published: 27 February 2020
(This article belongs to the Special Issue Micro and Nano Devices for Cell Analysis)
Both Young’s modulus and specific membrane capacitance (SMC) are two important physical parameters for characterizing cell status. In this paper, we utilized a thin-neck-micropipette aspiration system to simultaneously quantify Young’s modulus and SMC value of six types of cell lines in different progression grades, which include four grades from the lowest metastatic potential G1 to the highest potential G4. We investigated how these two physical properties possess heterogeneities in bladder cancer cells with different grades and what roles they might play in grading bladder cancer. The characterization results of these cells of different cancer grades is linearly correlated with the cancer grades, showing that the Young’s modulus is negatively linearly correlated with bladder cancer grades, while SMC shows a positive linear correlation. Furthermore, the combination of these two physical properties on a scatter diagram clearly shows the cell groups with different cancer grades, which means that this combination could be a potential tumor grading marker to identify cancer cells with different metastatic potential. View Full-Text
Keywords: single cell analysis; bladder cancer; micropipette aspiration; Young’s modulus; specific membrane capacitance single cell analysis; bladder cancer; micropipette aspiration; Young’s modulus; specific membrane capacitance
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Liu, N.; Leng, M.; Yue, T.; Dong, L.; Liu, Y.; Peng, Y.; Pu, H.; Xie, S.; Luo, J. Simultaneously Quantifying Both Young’s Modulus and Specific Membrane Capacitance of Bladder Cancer Cells with Different Metastatic Potential. Micromachines 2020, 11, 249.

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