Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization

Pitingolo, Gabriele; Riaud, Antoine; Nastruzzi, Claudio; Taly, Valerie

doi:10.3390/mi10040265

Open AccessCommunication

Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization

by

Gabriele Pitingolo

^1,*,†

,

Antoine Riaud

¹

,

Claudio Nastruzzi

² and

Valerie Taly

^1,*

¹

INSERM UMR-S1147, CNRS SNC5014, Paris Descartes University, Equipe Labellisée Ligue Nationale Contre le Cancer, 75005 Paris, France

²

Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Ferrara, 44121 Ferrara, Italy

^*

Authors to whom correspondence should be addressed.

^†

Present address: Département de Chimie, UMR 8640 PASTEUR, Ecole Normale Supérieure-PSL Research University, 75005 Paris, France.

Micromachines 2019, 10(4), 265; https://doi.org/10.3390/mi10040265

Submission received: 19 March 2019 / Revised: 2 April 2019 / Accepted: 9 April 2019 / Published: 19 April 2019

(This article belongs to the Special Issue Micro/Nano-system for Drug Delivery)

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Abstract

Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to test new drugs. Here, we report the use of a dehydrated gelatin film to promote tumor cells aggregation and 3D microtissue formation. The simple and stable gelatin coating represents an alternative to conventional and expensive materials like type I collagen, hyaluronic acid, or matrigel. The gelatin coating is biocompatible with several culture formats including microfluidic chips, as well as standard micro-well plates. It also enables long-term 3D cell culture and in situ monitoring of live/dead assays.

Keywords: 3D microtissues; microfluidics; human colon adenocarcinoma; cells viability; dehydration; gelatin; hydrogel

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MDPI and ACS Style

Pitingolo, G.; Riaud, A.; Nastruzzi, C.; Taly, V. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines 2019, 10, 265. https://doi.org/10.3390/mi10040265

AMA Style

Pitingolo G, Riaud A, Nastruzzi C, Taly V. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines. 2019; 10(4):265. https://doi.org/10.3390/mi10040265

Chicago/Turabian Style

Pitingolo, Gabriele, Antoine Riaud, Claudio Nastruzzi, and Valerie Taly. 2019. "Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization" Micromachines 10, no. 4: 265. https://doi.org/10.3390/mi10040265

APA Style

Pitingolo, G., Riaud, A., Nastruzzi, C., & Taly, V. (2019). Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines, 10(4), 265. https://doi.org/10.3390/mi10040265

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization

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