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Open AccessArticle

Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer

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Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia
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Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia
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Author to whom correspondence should be addressed.
Micromachines 2019, 10(11), 750; https://doi.org/10.3390/mi10110750
Received: 3 October 2019 / Revised: 27 October 2019 / Accepted: 31 October 2019 / Published: 1 November 2019
(This article belongs to the Special Issue Micro/Nano-system for Drug Delivery)
Extracellular vesicles provide cell-to-cell communication and have great potential for use as therapeutic carriers. This study was aimed at the development of an extracellular vesicle-based system for nucleic acid delivery. Three types of nanovesicles were assayed as oligonucleotide carriers: mesenchymal stem cell-derived extracellular vesicles and mimics prepared either by cell treatment with cytochalasin B or by vesicle generation from plasma membrane. Nanovesicles were loaded with a DNA oligonucleotide by freezing/thawing, sonication, or permeabilization with saponin. Oligonucleotide delivery was assayed using HEK293 cells. Extracellular vesicles and mimics were characterized by a similar oligonucleotide loading level but different efficiency of oligonucleotide delivery. Cytochalasin-B-inducible nanovesicles exhibited the highest level of oligonucleotide accumulation in HEK293 cells and a loading capacity of 0.44 ± 0.05 pmol/µg. The loaded oligonucleotide was mostly protected from nuclease action.
Keywords: extracellular vesicles (EVs), cytochalasin B; mesenchymal stem cells (MSCs), nucleic acid delivery; nanovesicles; freezing and thawing extracellular vesicles (EVs), cytochalasin B; mesenchymal stem cells (MSCs), nucleic acid delivery; nanovesicles; freezing and thawing
MDPI and ACS Style

Oshchepkova, A.; Neumestova, A.; Matveeva, V.; Artemyeva, L.; Morozova, K.; Kiseleva, E.; Zenkova, M.; Vlassov, V. Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer. Micromachines 2019, 10, 750.

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