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Article

Acute Toxicity of the Recombinant and Native Phα1β Toxin: New Analgesic from Phoneutria nigriventer Spider Venom

1
Department of Pharmacosciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170 Brazil
2
Postgraduate Program in Genetics and Applied Toxicology, Lutheran University of Brazil, Canoas, RS 92425–900, Brazil
3
Department of Pharmacology, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90050-17, Brazil
4
Postgraduate Program in Cellular and Molecular Biology Applied of Health, Lutheran University of Brazil, Canoas, RS 92425–900, Brazil
5
Postgraduate Program in Health Sciences: Medicine and Biomedicine, Institute of Education and Research, Grupo Santa Casa de Belo Horizonte, Belo Horizonte, MG 30150-240, Brazil
*
Author to whom correspondence should be addressed.
Toxins 2018, 10(12), 531; https://doi.org/10.3390/toxins10120531
Received: 24 October 2018 / Revised: 7 November 2018 / Accepted: 13 November 2018 / Published: 12 December 2018
Phα1β, a purified peptide from the venom of the spider Phoneutria nigriventer, and its recombinant form CTK 01512-2 are voltage-dependent calcium channel (CaV) blockers of types N, R, P/Q, and L with a preference for type N. These peptides show analgesic action in different pain models in rats. The aim of this study was to evaluate the acute intrathecal toxicity of the native and recombinant Phα1β toxin in Wistar rats. Clinical signs, serum biochemistry, organ weight, and histopathological alterations were evaluated in male and/or female rats. Dyspnea was observed in males, hyporesponsiveness in females, and Straub tail and tremors in both genders. There were no significant differences in male organ weight, although significant differences in the female relative weight of the adrenal glands and spleen have been observed; these values are within the normal range. Serum biochemical data revealed a significant reduction within the physiological limits of species related to urea, ALT, AST, and FA. Hepatic and renal congestion were observed for toxin groups. In renal tissue, glomerular infiltrates were observed with increased glomerular space. These histological alterations were presented in focal areas and in mild degree. Therefore, Phα1β and CTK 01512-2 presented a good safety profile with transient toxicity clinical signals in doses higher than used to obtain the analgesic effect. View Full-Text
Keywords: Phoneutria nigriventer; Phα1β; acute toxicity Phoneutria nigriventer; Phα1β; acute toxicity
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MDPI and ACS Style

Dallegrave, E.; Taschetto, E.; Bainy Leal, M.; Techera Antunes, F.T.; Gomez, M.V.; Hubner de Souza, A. Acute Toxicity of the Recombinant and Native Phα1β Toxin: New Analgesic from Phoneutria nigriventer Spider Venom. Toxins 2018, 10, 531. https://doi.org/10.3390/toxins10120531

AMA Style

Dallegrave E, Taschetto E, Bainy Leal M, Techera Antunes FT, Gomez MV, Hubner de Souza A. Acute Toxicity of the Recombinant and Native Phα1β Toxin: New Analgesic from Phoneutria nigriventer Spider Venom. Toxins. 2018; 10(12):531. https://doi.org/10.3390/toxins10120531

Chicago/Turabian Style

Dallegrave, Eliane, Eliane Taschetto, Mirna Bainy Leal, Flavia Tasmim Techera Antunes, Marcus Vinicius Gomez, and Alessandra Hubner de Souza. 2018. "Acute Toxicity of the Recombinant and Native Phα1β Toxin: New Analgesic from Phoneutria nigriventer Spider Venom" Toxins 10, no. 12: 531. https://doi.org/10.3390/toxins10120531

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