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Toxins 2018, 10(11), 484; https://doi.org/10.3390/toxins10110484

Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents Using an In Vitro Multicompartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry

1
Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Real de Juriquilla, Queretaro 76230, Mexico
2
National Autonomous University of Mexico-Superior Studies Faculty at Cuautitlan (UNAM–FESC), Multidisciplinary Research Unit L5, Cuautitlan Izcalli 54714, Mexico
3
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
4
National Autonomous University of Mexico-Superior Studies Faculty at Cuautitlan (UNAM–FESC), Multidisciplinary Research Unit L14 (Food, Mycotoxins, and Mycotoxicosis), Cuautitlan Izcalli 54714, Mexico
These authors have contributed equally to the work.
*
Author to whom correspondence should be addressed.
Received: 5 November 2018 / Revised: 17 November 2018 / Accepted: 18 November 2018 / Published: 21 November 2018
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Abstract

Experiments were carried out to evaluate the effectiveness of three different biosorbents (banana peel, Pyracantha leaves, and Aloe powder) in removing aflatoxin B1 (AFB1). A noncommercial mycotoxin binder (zeolite) was used as a reference material. A laboratory model that simulated the in vivo conditions of the poultry gastrointestinal tract was utilized to prove the removal efficiency of the biosorbents when added to AFB1-contaminated diet (100 µg/kg). The concentration of AFB1 was determined using antibody-based immunoaffinity column and spectrofluorometry methodologies. Z potential (ζ), point of zero charge (pHpzc), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR), and UV-Vis diffuse reflectance spectroscopy (DRS) techniques were used to further characterize the biosorbents. The addition of the biosorbents (1.5%, w/w) to the diet significantly reduced the bioavailability of AFB1 in the intestinal section. The highest aflatoxin adsorption values were 69% and 70% using Aloe powder and zeolite, respectively. A moderate biosorption uptake of 46% was achieved using Pyracantha leaves. The biomaterial with the lowest removal capacity was banana peel (28%). In conclusion, Aloe powder could be used as an alternative to conventional systems for AFB1 removal. View Full-Text
Keywords: aflatoxin B1; decontamination; biosorbents; in vitro digestion model aflatoxin B1; decontamination; biosorbents; in vitro digestion model
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zavala-Franco, A.; Hernández-Patlán, D.; Solís-Cruz, B.; López-Arellano, R.; Tellez-Isaias, G.; Vázquez-Durán, A.; Méndez-Albores, A. Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents Using an In Vitro Multicompartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry. Toxins 2018, 10, 484.

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