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Toxins 2018, 10(11), 485; https://doi.org/10.3390/toxins10110485

Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum

1
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
2
Department of Chemistry and Bioscience, Aalborg University, 6700 Esbjerg, Denmark
*
Author to whom correspondence should be addressed.
Received: 30 October 2018 / Revised: 15 November 2018 / Accepted: 18 November 2018 / Published: 21 November 2018
(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)
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Abstract

There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in Fusarium graminearum. Proteomic analyses showed that overexpression of AurR1 resulted in a significant increase of five of the eleven proteins belonging to the aurofusarin biosynthetic pathway. Further, the production of aurofusarin was increased more than threefold in the overexpression mutant compared to the wild type, reaching levels of 270 mg/L. In addition to biosynthesis of aurofusarin, several yet undescribed putative naphthoquinone/anthraquinone analogue compounds were observed in the overexpression mutant. Our results suggest that it is possible to enhance the aurofusarin production through genetic engineering. View Full-Text
Keywords: aurofusarin; PKS12; secondary metabolites; polyketide; natural products; aurR1; Fusarium; pigment; fungi; mycotoxins aurofusarin; PKS12; secondary metabolites; polyketide; natural products; aurR1; Fusarium; pigment; fungi; mycotoxins
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Westphal, K.R.; Wollenberg, R.D.; Herbst, F.-A.; Sørensen, J.L.; Sondergaard, T.E.; Wimmer, R. Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum. Toxins 2018, 10, 485.

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