Next Article in Journal
The Acute Toxicity of Tetrodotoxin and Tetrodotoxin–Saxitoxin Mixtures to Mice by Various Routes of Administration
Next Article in Special Issue
Multi-(myco)toxins in Malting and Brewing By-Products
Previous Article in Journal
Variations in the Botulinum Neurotoxin Binding Domain and the Potential for Novel Therapeutics
Previous Article in Special Issue
Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 10
Issue 11
10.3390/toxins10110422
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Detection of Shiga Toxin 2 Produced by Escherichia coli in Foods Using a Novel AlphaLISA

by
Cheryl M. Armstrong
1,
Leah E. Ruth
2,
Joseph A. Capobianco
1,
Terence P. Strobaugh, Jr.
1,
Fernando M. Rubio
2 and
Andrew G. Gehring
1,*
1
Molecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
2
Abraxis, Inc., Warminster, PA 18974, USA
*
Author to whom correspondence should be addressed.
Toxins 2018, 10(11), 422; https://doi.org/10.3390/toxins10110422
Submission received: 14 September 2018 / Revised: 9 October 2018 / Accepted: 17 October 2018 / Published: 23 October 2018
(This article belongs to the Special Issue Food Safety and Natural Toxins)
Browse Figures
Versions Notes

Abstract

Amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) is comprised of a bead-based immunoassay that is used for small molecule detection. In this study, a novel AlphaLISA was developed and optimized for the detection of Shiga-toxin 2 (Stx2). Efficacy and sensitivity trials showed the AlphaLISA could detect ≥0.5 ng/mL of purified Stx2, which was comparable to the industry-standard enzyme-linked immunosorbent assay (ELISA) tests for Stx2 detection. In addition, evaluation of Shiga toxin-producing Escherichia coli (STEC)-inoculated Romaine lettuce and ground beef samples demonstrated that both the AlphaLISA and the ELISA were able to discern uninoculated samples from 1× and 10× diluted samples containing ~10 CFU/mL of STEC enriched in modified tryptic soy broth with mitomycin C for 16 h. Overall, the increased signal-to-noise ratios indicated a more robust signal was produced by the AlphaLISA compared to the ELISA and the delineation of higher toxin concentrations without the need for sample dilution implied a greater dynamic range for the AlphaLISA. Implementation of the newly developed AlphaLISA will allow for more rapid analysis for Stx2 with less manual manipulation, thus improving assay throughput and the ability to automate sample screening while maintaining detection limits of 0.5 ng/mL.
Keywords: amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA); detection; enzyme-linked immunosorbent assay (ELISA); E. coli; Shiga toxin; STEC; Stx2 amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA); detection; enzyme-linked immunosorbent assay (ELISA); E. coli; Shiga toxin; STEC; Stx2

Share and Cite

MDPI and ACS Style

Armstrong, C.M.; Ruth, L.E.; Capobianco, J.A.; Strobaugh, T.P., Jr.; Rubio, F.M.; Gehring, A.G. Detection of Shiga Toxin 2 Produced by Escherichia coli in Foods Using a Novel AlphaLISA. Toxins 2018, 10, 422. https://doi.org/10.3390/toxins10110422

AMA Style

Armstrong CM, Ruth LE, Capobianco JA, Strobaugh TP Jr., Rubio FM, Gehring AG. Detection of Shiga Toxin 2 Produced by Escherichia coli in Foods Using a Novel AlphaLISA. Toxins. 2018; 10(11):422. https://doi.org/10.3390/toxins10110422

Chicago/Turabian Style

Armstrong, Cheryl M., Leah E. Ruth, Joseph A. Capobianco, Terence P. Strobaugh, Jr., Fernando M. Rubio, and Andrew G. Gehring. 2018. "Detection of Shiga Toxin 2 Produced by Escherichia coli in Foods Using a Novel AlphaLISA" Toxins 10, no. 11: 422. https://doi.org/10.3390/toxins10110422

APA Style

Armstrong, C. M., Ruth, L. E., Capobianco, J. A., Strobaugh, T. P., Jr., Rubio, F. M., & Gehring, A. G. (2018). Detection of Shiga Toxin 2 Produced by Escherichia coli in Foods Using a Novel AlphaLISA. Toxins, 10(11), 422. https://doi.org/10.3390/toxins10110422

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop