Manuka Honey Inhibits Human Breast Cancer Progression in Preclinical Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Lines
2.3. Cell Proliferation Assay
2.4. Western Blot Analysis
2.5. Annexin V Staining Assay
2.6. Human Tumor Xenografts in Nude Mice
2.7. Statistics
3. Results
3.1. Manuka Honey Reduces the Proliferation of Human MCF-7 Breast Cancer Cells In Vitro
3.2. MH Induces Apoptosis in MCF-7 Breast Cancer Cells In Vitro
3.3. Manuka Honey Induces PARP-Cleavage Leading to Tumor Cell Apoptosis
3.4. Manuka Honey Activates AMPK and Inhibits Downstream PI3K/AKT/mTOR Signaling
3.5. Manuka Honey Inhibits STAT-3 Phosphorylation
3.6. Antitumor Activity of Manuka Honey in Human Breast Cancer Xenografts In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Márquez-Garbán, D.C.; Yanes, C.D.; Llarena, G.; Elashoff, D.; Hamilton, N.; Hardy, M.; Wadehra, M.; McCloskey, S.A.; Pietras, R.J. Manuka Honey Inhibits Human Breast Cancer Progression in Preclinical Models. Nutrients 2024, 16, 2369. https://doi.org/10.3390/nu16142369
Márquez-Garbán DC, Yanes CD, Llarena G, Elashoff D, Hamilton N, Hardy M, Wadehra M, McCloskey SA, Pietras RJ. Manuka Honey Inhibits Human Breast Cancer Progression in Preclinical Models. Nutrients. 2024; 16(14):2369. https://doi.org/10.3390/nu16142369
Chicago/Turabian StyleMárquez-Garbán, Diana C., Cristian D. Yanes, Gabriela Llarena, David Elashoff, Nalo Hamilton, Mary Hardy, Madhuri Wadehra, Susan A. McCloskey, and Richard J. Pietras. 2024. "Manuka Honey Inhibits Human Breast Cancer Progression in Preclinical Models" Nutrients 16, no. 14: 2369. https://doi.org/10.3390/nu16142369