Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Digestion Experiments
2.3. Human Intervention Study
2.4. High-Pressure Liquid Chromatography with UV Detection (HPLC–UV) of MGO after Derivatization to Quinoxalines
2.5. High-Pressure Liquid Chromatography with Tandem Mass Spectrometric (HPLC–MS/MS) Detection
2.6. Statistical Treatment
3. Results
3.1. Methylglyoxal (MGO) Reacts with Lysine, Arginine, and Creatine during Simulated Digestion
3.2. Reaction of MGO and Creatine during Simultaneous Digestion in Human Volunteers
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Transition | Fragmentor Voltage [V] | Collision Energy [eV] | Dwell Time [ms] | Q/q 1 | |
---|---|---|---|---|---|
MG-HCr | 186 → 87 | 110 | 20 | 180 | Q |
186 → 44 | 110 | 25 | 180 | q | |
D3-MG-HCr | 189 → 90 | 110 | 20 | 130 | Q |
189 → 44 | 110 | 25 | 130 | q | |
CEL | 219 → 130 | 100 | 10 | 70 | q |
219 → 84 | 100 | 20 | 70 | Q | |
13C3-CEL | 222 → 130 | 90 | 10 | 70 | q |
222 → 84 | 90 | 20 | 70 | Q | |
Creatine | 132 → 90 | 75 | 10 | 60 | Q |
132 → 44 | 75 | 20 | 60 | q | |
D3-Creatine | 135 → 93 | 90 | 10 | 60 | Q |
135 → 47 | 90 | 20 | 60 | q | |
Creatinine | 114 → 86 | 105 | 10 | 50 | Q |
114 → 44 | 105 | 14 | 50 | q | |
D3-Creatinine | 117 → 89 | 105 | 10 | 50 | Q |
117 → 47 | 105 | 14 | 50 | q | |
MG-H1 | 229 → 166 | 75 | 10 | 120 | q |
229 → 114 | 75 | 10 | 120 | Q | |
13C6-MG-H1 | 235 → 171 | 120 | 10 | 120 | q |
235 → 115 | 120 | 10 | 120 | Q |
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Treibmann, S.; Groß, J.; Pätzold, S.; Henle, T. Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers. Nutrients 2022, 14, 3598. https://doi.org/10.3390/nu14173598
Treibmann S, Groß J, Pätzold S, Henle T. Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers. Nutrients. 2022; 14(17):3598. https://doi.org/10.3390/nu14173598
Chicago/Turabian StyleTreibmann, Stephanie, Julia Groß, Susann Pätzold, and Thomas Henle. 2022. "Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers" Nutrients 14, no. 17: 3598. https://doi.org/10.3390/nu14173598