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Article

Synbiotic Compositions of Bacillus megaterium and Polyunsaturated Fatty Acid Salt Enable Self-Sufficient Production of Specialized Pro-Resolving Mediators

1
Evonik Operations GmbH, Rodenbacher Chaussee 4, 63457 Hanau, Germany
2
Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, 07743 Jena, Germany
3
BioTeSys GmbH, Schelztorstraße 54-56, 73728 Esslingen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen Cornish
Nutrients 2022, 14(11), 2265; https://doi.org/10.3390/nu14112265
Received: 5 May 2022 / Revised: 23 May 2022 / Accepted: 25 May 2022 / Published: 28 May 2022
Specialized pro-resolving mediators (SPM) have emerged as crucial lipid mediators that confer the inflammation-resolving effects of omega-3 polyunsaturated fatty acids (n-3 PUFA). Importantly, SPM biosynthesis is dysfunctional in various conditions, which may explain the inconclusive efficacy data from n-3 PUFA interventions. To overcome the limitations of conventional n-3 PUFA supplementation strategies, we devised a composition enabling the self-sufficient production of SPM in vivo. Bacillus megaterium strains were fed highly bioavailable n-3 PUFA, followed by metabololipidomics analysis and bioinformatic assessment of the microbial genomes. All 48 tested Bacillus megaterium strains fed with the n-3 PUFA formulation produced a broad range of SPM and precursors thereof in a strain-specific manner, which may be explained by the CYP102A1 gene polymorphisms that we detected. A pilot study was performed to test if a synbiotic Bacillus megaterium/n-3 PUFA formulation increases SPM levels in vivo. Supplementation with a synbiotic capsule product led to significantly increased plasma levels of hydroxy-eicosapentaenoic acids (5-HEPE, 15-HEPE, 18-HEPE) and hydroxy-docosahexaenoic acids (4-HDHA, 7-HDHA) as well as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in healthy humans. To the best of our knowledge, we report here for the first time the development and in vivo application of a self-sufficient SPM-producing formulation. Further investigations are warranted to confirm and expand these findings, which may create a new class of n-3 PUFA interventions targeting inflammation resolution. View Full-Text
Keywords: probiotic; CYP450BM3; dietary supplement; drug; inflammatory bowel diseases; irritable bowel syndrome; n-3 PUFA; SPM probiotic; CYP450BM3; dietary supplement; drug; inflammatory bowel diseases; irritable bowel syndrome; n-3 PUFA; SPM
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MDPI and ACS Style

Speckmann, B.; Kleinbölting, J.; Börner, F.; Jordan, P.M.; Werz, O.; Pelzer, S.; tom Dieck, H.; Wagner, T.; Schön, C. Synbiotic Compositions of Bacillus megaterium and Polyunsaturated Fatty Acid Salt Enable Self-Sufficient Production of Specialized Pro-Resolving Mediators. Nutrients 2022, 14, 2265. https://doi.org/10.3390/nu14112265

AMA Style

Speckmann B, Kleinbölting J, Börner F, Jordan PM, Werz O, Pelzer S, tom Dieck H, Wagner T, Schön C. Synbiotic Compositions of Bacillus megaterium and Polyunsaturated Fatty Acid Salt Enable Self-Sufficient Production of Specialized Pro-Resolving Mediators. Nutrients. 2022; 14(11):2265. https://doi.org/10.3390/nu14112265

Chicago/Turabian Style

Speckmann, Bodo, Jessica Kleinbölting, Friedemann Börner, Paul M. Jordan, Oliver Werz, Stefan Pelzer, Heike tom Dieck, Tanja Wagner, and Christiane Schön. 2022. "Synbiotic Compositions of Bacillus megaterium and Polyunsaturated Fatty Acid Salt Enable Self-Sufficient Production of Specialized Pro-Resolving Mediators" Nutrients 14, no. 11: 2265. https://doi.org/10.3390/nu14112265

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