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Article

The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats

1
Department of Pharmacology and Toxicology, UWM, 10-082 Olsztyn, Poland
2
Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland
3
Department of Mental and Psychosomatic Diseases, Faculty of Medicine, UWM, 10-228 Olsztyn, Poland
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Department of Cardiology and Internal Medicine, Faculty of Medicine, UWM, 10-082 Olsztyn, Poland
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Department of Bromatology, Medical University of Białystok, 15-222 Białystok, Poland
6
Department of Biochemistry and Toxicology, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences, 20-950 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Gaetano Santulli and Bruno Trimarco
Nutrients 2021, 13(11), 3793; https://doi.org/10.3390/nu13113793
Received: 25 September 2021 / Revised: 21 October 2021 / Accepted: 24 October 2021 / Published: 26 October 2021
(This article belongs to the Special Issue Dietary Supplements in Cardiovascular and Metabolic Diseases)
Recently, the addition of copper nanoparticles (NPs) in a daily diet (6.5 mg/kg) was studied in different animal models as a possible alternative to ionic forms. Male Wistar–Kyoto rats (24-week-old, n = 11) were fed with copper, either in the form of carbonate salt (Cu6.5) or metal-based copper NPs (NP6.5), for 8 weeks. The third group was fed with a half dose of each (NP3.25 + Cu3.25). The thoracic aorta and blood plasma was studied. Supplementation with NP6.5 decreased the Cu (×0.7), Cu/Zn-ratio (×0.6) and catalase (CAT, ×0.7), and increased Zn (×1.2) and superoxide dismutase (SOD, ×1.4). Meanwhile, NP3.25 + Cu3.25 decreased the Cu/Zn-ratio (×0.7), and CAT (×0.7), and increased the daily feed intake (×1.06). Preincubation with either the selective cyclooxygenase (COX)-2 inhibitor, or the non-selective COX-1/2 inhibitor attenuated vasodilation of rat thoracic aorta in the NP6.5 group exclusively. However, an increased vasodilator response was observed in the NP6.5 and NP3.25 + Cu3.25 group of rats after preincubation with an inhibitor of 20-hydroxyeicosatetraenoic acid (20-HETE) formation, and the thromboxane receptor (TP) antagonist. Significant differences were observed between the NP6.5 and NP3.25 + Cu3.25 groups of rats in: dietary intake, acetylcholine-induced vasodilation, and response to COX-inhibitors. Copper NPs in a standard daily dose had more significant effects on the mechanism(s) responsible for the utilization of reactive oxygen species in the blood plasma with the participation of prostanoids derived from COX-2 in the vascular relaxation. Dietary copper NPs in both doses modified vasodilation through the vasoconstrictor 20-HETE and the TP receptors. View Full-Text
Keywords: aging; 20-HETE; furegrelate; HET0016; indomethacin; NS-398; SQ-29,548; thromboxane-A2 aging; 20-HETE; furegrelate; HET0016; indomethacin; NS-398; SQ-29,548; thromboxane-A2
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MDPI and ACS Style

Majewski, M.; Juśkiewicz, J.; Krajewska-Włodarczyk, M.; Gromadziński, L.; Socha, K.; Cholewińska, E.; Ognik, K. The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats. Nutrients 2021, 13, 3793. https://doi.org/10.3390/nu13113793

AMA Style

Majewski M, Juśkiewicz J, Krajewska-Włodarczyk M, Gromadziński L, Socha K, Cholewińska E, Ognik K. The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats. Nutrients. 2021; 13(11):3793. https://doi.org/10.3390/nu13113793

Chicago/Turabian Style

Majewski, Michał, Jerzy Juśkiewicz, Magdalena Krajewska-Włodarczyk, Leszek Gromadziński, Katarzyna Socha, Ewelina Cholewińska, and Katarzyna Ognik. 2021. "The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats" Nutrients 13, no. 11: 3793. https://doi.org/10.3390/nu13113793

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