PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults

Grubb, Diana S.; Wrigley, Scott D.; Freedman, Kimberley E.; Wei, Yuren; Vazquez, Allegra R.; Trotter, Roxanne E.; Wallace, Taylor C.; Johnson, Sarah A.; Weir, Tiffany L.

doi:10.3390/nu12082474

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PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults

Diana S. Grubb

¹,

Scott D. Wrigley

¹,

Kimberley E. Freedman

¹,

¹,

^1,2,

¹,

^3,4

² and

^1,*

Intestinal Health Laboratory, Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO 80523, USA

Functional Foods & Human Health Laboratory, Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO 80523, USA

Think Healthy Group, 1301 20th Street, NW, #413, Washington, DC 20036, USA

⁴

Department of Nutrition and Food Studies, George Mason University, 4400 University Drive, MS:1F7, Fairfax, VA 22030, USA

Author to whom correspondence should be addressed.

Nutrients 2020, 12(8), 2474; https://doi.org/10.3390/nu12082474

Received: 15 July 2020 / Revised: 12 August 2020 / Accepted: 13 August 2020 / Published: 17 August 2020

(This article belongs to the Special Issue Role of Prebiotics and Probiotics in Health and Disease)

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Abstract

Probiotics are increasingly used by consumers and practitioners to reduce gastrointestinal (GI) distress and improve gut function. Here, we sought to determine whether the addition of supplemental bacteriophages (PreforPro) could enhance the effects of a common probiotic, Bifidobacterium animalis subsp. lactis (B. lactis) on GI health. A total of 68 participants were enrolled in a 4-week, randomized, parallel-arm, double-blind, placebo-controlled trial where primary outcomes included self-assessments of GI health, a daily stool log, and 16s rRNA analysis of gut microbial populations. We observed within-group improvements in GI inflammation (p = 0.01) and a trending improvement in colon pain (p = 0.08) in individuals consuming B. lactis with PreforPro, but not in the group consuming only the probiotic. There was also a larger increase in Lactobacillus and short-chain fatty acid-producing microbial taxa detected in the stool of participants taking PreforPro with B. lactis compared to the probiotic alone. Overall, these results suggest the addition of PreforPro as a combination therapy may alter gut ecology to extend the GI benefits of consuming B. lactis or other probiotics. View Full-Text

Keywords: bacteriophage; Bifidobacterium; gut microbiota; intestinal health; microbiome; probiotic

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MDPI and ACS Style

Grubb, D.S.; Wrigley, S.D.; Freedman, K.E.; Wei, Y.; Vazquez, A.R.; Trotter, R.E.; Wallace, T.C.; Johnson, S.A.; Weir, T.L. PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults. Nutrients 2020, 12, 2474. https://doi.org/10.3390/nu12082474

AMA Style

Grubb DS, Wrigley SD, Freedman KE, Wei Y, Vazquez AR, Trotter RE, Wallace TC, Johnson SA, Weir TL. PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults. Nutrients. 2020; 12(8):2474. https://doi.org/10.3390/nu12082474

Chicago/Turabian Style

Grubb, Diana S., Scott D. Wrigley, Kimberley E. Freedman, Yuren Wei, Allegra R. Vazquez, Roxanne E. Trotter, Taylor C. Wallace, Sarah A. Johnson, and Tiffany L. Weir. 2020. "PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults" Nutrients 12, no. 8: 2474. https://doi.org/10.3390/nu12082474

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PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota in Healthy Adults

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