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Article

Preclinical Evaluation of a Food-Derived Functional Ingredient to Address Skeletal Muscle Atrophy

Nuritas Limited, Dublin D02 RY95, Ireland
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Nutrients 2020, 12(8), 2274; https://doi.org/10.3390/nu12082274
Received: 3 July 2020 / Revised: 23 July 2020 / Accepted: 24 July 2020 / Published: 29 July 2020
(This article belongs to the Special Issue Nutrition for Human Health, Performance and Recovery)
Skeletal muscle is the metabolic powerhouse of the body, however, dysregulation of the mechanisms involved in skeletal muscle mass maintenance can have devastating effects leading to many metabolic and physiological diseases. The lack of effective solutions makes finding a validated nutritional intervention an urgent unmet medical need. In vitro testing in murine skeletal muscle cells and human macrophages was carried out to determine the effect of a hydrolysate derived from vicia faba (PeptiStrong: NPN_1) against phosphorylated S6, atrophy gene expression, and tumour necrosis factor alpha (TNF-α) secretion, respectively. Finally, the efficacy of NPN_1 on attenuating muscle waste in vivo was assessed in an atrophy murine model. Treatment of NPN_1 significantly increased the phosphorylation of S6, downregulated muscle atrophy related genes, and reduced lipopolysaccharide-induced TNF-α release in vitro. In a disuse atrophy murine model, following 18 days of NPN_1 treatment, mice exhibited a significant attenuation of muscle loss in the soleus muscle and increased the integrated expression of Type I and Type IIa fibres. At the RNA level, a significant upregulation of protein synthesis-related genes was observed in the soleus muscle following NPN_1 treatment. In vitro and preclinical results suggest that NPN_1 is an effective bioactive ingredient with great potential to prolong muscle health. View Full-Text
Keywords: protein synthesis; muscle atrophy; inflammation; skeletal muscle; aging; immobilization; peptide; bioactive; functional ingredient protein synthesis; muscle atrophy; inflammation; skeletal muscle; aging; immobilization; peptide; bioactive; functional ingredient
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MDPI and ACS Style

Cal, R.; Davis, H.; Kerr, A.; Wall, A.; Molloy, B.; Chauhan, S.; Trajkovic, S.; Holyer, I.; Adelfio, A.; Khaldi, N. Preclinical Evaluation of a Food-Derived Functional Ingredient to Address Skeletal Muscle Atrophy. Nutrients 2020, 12, 2274. https://doi.org/10.3390/nu12082274

AMA Style

Cal R, Davis H, Kerr A, Wall A, Molloy B, Chauhan S, Trajkovic S, Holyer I, Adelfio A, Khaldi N. Preclinical Evaluation of a Food-Derived Functional Ingredient to Address Skeletal Muscle Atrophy. Nutrients. 2020; 12(8):2274. https://doi.org/10.3390/nu12082274

Chicago/Turabian Style

Cal, Roi, Heidi Davis, Alish Kerr, Audrey Wall, Brendan Molloy, Sweeny Chauhan, Sanja Trajkovic, Ian Holyer, Alessandro Adelfio, and Nora Khaldi. 2020. "Preclinical Evaluation of a Food-Derived Functional Ingredient to Address Skeletal Muscle Atrophy" Nutrients 12, no. 8: 2274. https://doi.org/10.3390/nu12082274

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