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Article

The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia

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Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, Austria
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Department of Biomedical Research, Medical University of Graz, 8036 Graz, Austria
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Core Facility Molecular Biology, Center for Medical Research, Medical University of Graz, 8036 Graz, Austria
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Division of Paediatric Haematology and Oncology, Department of Pediatrics and Adolescent Medicine, Medical University of Graz, 8036 Graz, Austria
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Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria
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Center for Biomarker Research in Medicine (CBmed), 8036 Graz, Austria
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Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anesthesiology and Intensive Care, Rostock University Medical Center, 18057 Rostock, Germany
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Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
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Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, 8036 Graz, Austria
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(7), 2029; https://doi.org/10.3390/nu12072029
Received: 5 June 2020 / Revised: 26 June 2020 / Accepted: 4 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Role of Prebiotics and Probiotics in Health and Disease)
Malignant diseases can cause tumor-associated cachexia (TAC). Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases. The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC. For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12). Three animals of each tumor group did not develop NB. The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791). At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured. Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10). Alpha diversity did not differ significantly between the groups. Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals. Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome. Of 49 VOCs, 22 showed significant differences between the groups. NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes. The pore and leak pathways of tight junctions did not differ between groups. In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile. Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability. View Full-Text
Keywords: prebiotics; gut permeability; neuroblastoma; microbiome; volatile organic compounds prebiotics; gut permeability; neuroblastoma; microbiome; volatile organic compounds
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MDPI and ACS Style

Obermüller, B.; Singer, G.; Kienesberger, B.; Klymiuk, I.; Sperl, D.; Stadlbauer, V.; Horvath, A.; Miekisch, W.; Gierschner, P.; Grabherr, R.; Gruber, H.-J.; Semeraro, M.D.; Till, H.; Castellani, C. The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia. Nutrients 2020, 12, 2029. https://doi.org/10.3390/nu12072029

AMA Style

Obermüller B, Singer G, Kienesberger B, Klymiuk I, Sperl D, Stadlbauer V, Horvath A, Miekisch W, Gierschner P, Grabherr R, Gruber H-J, Semeraro MD, Till H, Castellani C. The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia. Nutrients. 2020; 12(7):2029. https://doi.org/10.3390/nu12072029

Chicago/Turabian Style

Obermüller, Beate, Georg Singer, Bernhard Kienesberger, Ingeborg Klymiuk, Daniela Sperl, Vanessa Stadlbauer, Angela Horvath, Wolfram Miekisch, Peter Gierschner, Reingard Grabherr, Hans-Jürgen Gruber, Maria D. Semeraro, Holger Till, and Christoph Castellani. 2020. "The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia" Nutrients 12, no. 7: 2029. https://doi.org/10.3390/nu12072029

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