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Open AccessArticle

Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate

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Gut Microbes & Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich NR4 7UQ, UK
2
Earlham Institute, Norwich NR4 7UZ, UK
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Analytical Sciences, Quadram Institute Bioscience, Norwich NR4 7UQ, UK
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APC Microbiome Institute, University College Cork, T12 K8AF Cork, Ireland
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Lydia Becker Institute for Immunology and Inflammation, Wellcome Trust Centre for Cell Matrix Research, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PL, UK
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Norwich Medical School, University of East Anglia, Norwich NR4 7TJ, UK
*
Authors to whom correspondence should be addressed.
Nutrients 2020, 12(4), 948; https://doi.org/10.3390/nu12040948
Received: 10 January 2020 / Revised: 20 March 2020 / Accepted: 27 March 2020 / Published: 29 March 2020
(This article belongs to the Special Issue Impact of Dietary Components on Gut Microbiota)
Background: Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe–microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system. Methods: Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR). Results: Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel. Conclusions: These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health. View Full-Text
Keywords: Bifidobacterium; exopolysaccharides; early life; diet; model colon; cross-feeding; 16S rRNA profiling; metabolomics Bifidobacterium; exopolysaccharides; early life; diet; model colon; cross-feeding; 16S rRNA profiling; metabolomics
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MDPI and ACS Style

Püngel, D.; Treveil, A.; Dalby, M.J; Caim, S.; Colquhoun, I.J; Booth, C.; Ketskemety, J.; Korcsmaros, T.; van Sinderen, D.; Lawson, M.A.; Hall, L.J. Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate. Nutrients 2020, 12, 948. https://doi.org/10.3390/nu12040948

AMA Style

Püngel D, Treveil A, Dalby MJ, Caim S, Colquhoun IJ, Booth C, Ketskemety J, Korcsmaros T, van Sinderen D, Lawson MA, Hall LJ. Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate. Nutrients. 2020; 12(4):948. https://doi.org/10.3390/nu12040948

Chicago/Turabian Style

Püngel, Deborah; Treveil, Agatha; Dalby, Matthew J; Caim, Shabhonam; Colquhoun, Ian J; Booth, Catherine; Ketskemety, Jennifer; Korcsmaros, Tamas; van Sinderen, Douwe; Lawson, Melissa A.; Hall, Lindsay J 2020. "Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate" Nutrients 12, no. 4: 948. https://doi.org/10.3390/nu12040948

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