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Article

Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats

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Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland
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Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland
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Department of Future Technologies, University of Turku, FI-20014 Turku, Finland
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Institute of Biomedicine, University of Turku, FI-20014 Turku, Finland
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Department of Clinical Microbiology, Turku University Hospital, FI-20521 Turku, Finland
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Veterinary Pathology and Parasitology, University of Helsinki, FIN-00014 Helsinki, Finland
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Department of Environmental and Biological Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(11), 3225; https://doi.org/10.3390/nu12113225
Received: 7 September 2020 / Revised: 12 October 2020 / Accepted: 20 October 2020 / Published: 22 October 2020
(This article belongs to the Section Prebiotics and Probiotics)
Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice. Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, the effect of XOS on F. prausnitzii growth was assessed in vitro. Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group). XOS increased F. prausnitzii growth, having only a minor impact on the GM composition. When supplemented with HFD, XOS ameliorated hepatic steatosis. The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration. Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD. Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides. In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM. View Full-Text
Keywords: prebiotic; oligosaccharides; gut microbiota; fatty liver; metabolism; mitochondria prebiotic; oligosaccharides; gut microbiota; fatty liver; metabolism; mitochondria
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MDPI and ACS Style

Lensu, S.; Pariyani, R.; Mäkinen, E.; Yang, B.; Saleem, W.; Munukka, E.; Lehti, M.; Driuchina, A.; Lindén, J.; Tiirola, M.; Lahti, L.; Pekkala, S. Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats. Nutrients 2020, 12, 3225. https://doi.org/10.3390/nu12113225

AMA Style

Lensu S, Pariyani R, Mäkinen E, Yang B, Saleem W, Munukka E, Lehti M, Driuchina A, Lindén J, Tiirola M, Lahti L, Pekkala S. Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats. Nutrients. 2020; 12(11):3225. https://doi.org/10.3390/nu12113225

Chicago/Turabian Style

Lensu, Sanna, Raghunath Pariyani, Elina Mäkinen, Baoru Yang, Wisam Saleem, Eveliina Munukka, Maarit Lehti, Anastasiia Driuchina, Jere Lindén, Marja Tiirola, Leo Lahti, and Satu Pekkala. 2020. "Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats" Nutrients 12, no. 11: 3225. https://doi.org/10.3390/nu12113225

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