Next Article in Journal
Impact of Percent Body Fat on All-Cause Mortality among Adequate Dialysis Patients with and without Insulin Resistance: A Multi-Center Prospective Cohort Study
Previous Article in Journal
Prevention and Treatment of Sarcopenic Obesity in Women
Article Menu

Export Article

Open AccessArticle

Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain
Cembio (Centro de Metabolómica y Bioanálisis), Departamento de Química y Bioquímica, Facultad de Farmacia, Universidad CEU San Pablo, 28668 Monteprincipe, Spain
Authors to whom correspondence should be addressed.
Current address: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM) Nicolás Cabrera 9, 28049 Madrid, Spain.
Nutrients 2019, 11(6), 1303;
Received: 25 April 2019 / Revised: 3 June 2019 / Accepted: 4 June 2019 / Published: 8 June 2019
(This article belongs to the Special Issue Edible Fruits and Human Health)
PDF [1609 KB, uploaded 10 June 2019]


Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy. View Full-Text
Keywords: Allium porrum; antioxidant activity; phenolic compounds; mast cell; epithelium Allium porrum; antioxidant activity; phenolic compounds; mast cell; epithelium

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Benedé, S.; Gradillas, A.; Villalba, M.; Batanero, E. Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function. Nutrients 2019, 11, 1303.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top