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Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

1
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain
2
Cembio (Centro de Metabolómica y Bioanálisis), Departamento de Química y Bioquímica, Facultad de Farmacia, Universidad CEU San Pablo, 28668 Monteprincipe, Spain
*
Authors to whom correspondence should be addressed.
Current address: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM) Nicolás Cabrera 9, 28049 Madrid, Spain.
Nutrients 2019, 11(6), 1303; https://doi.org/10.3390/nu11061303
Received: 25 April 2019 / Revised: 3 June 2019 / Accepted: 4 June 2019 / Published: 8 June 2019
(This article belongs to the Special Issue Edible Fruits and Human Health)
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Abstract

Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy. View Full-Text
Keywords: Allium porrum; antioxidant activity; phenolic compounds; mast cell; epithelium Allium porrum; antioxidant activity; phenolic compounds; mast cell; epithelium
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Benedé, S.; Gradillas, A.; Villalba, M.; Batanero, E. Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function. Nutrients 2019, 11, 1303.

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