Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice
Abstract
:1. Introduction
2. Results
2.1. Lycii Radicis Cortex (LRC) and Achyrantes Japonica (AJ) Increased Osteoblast Differentiation and Mineralized Nodule Formation
2.2. LRC and AJ Increased mRNA Expression of Osteoblastic Markers
2.3. LRC and AJ Dcecreased Osteoclast Differentiation in Primary-Cultured Preosteoclast-Lineage Monocytes
2.4. LRC and AJ Inhibited OVX-Induced BMD Loss in Osteoporosis Model Mice
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Cell Proliferation Assay
4.3. Alkaline Phosphatase (ALP) Assay and Staining
4.4. Mineralized Nodule Formation
4.5. Quantitative Reverse-Transcription PCR (qRT-PCR)
4.6. In Vitro Generation of Osteoclasts
4.7. Ovariectomized Model
4.8. Measurement of Bone Loss in OVX Mice
4.9. Blood Sampling and Serum OPG and RANKL Measurement
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Park, E.; Kim, J.; Yeo, S.; Lim, E.; Choi, C.W.; Choi, S.; Li, W.Y.; Lee, J.-W.; Park, J.-H.; Huh, D.; et al. Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice. Nutrients 2019, 11, 2716. https://doi.org/10.3390/nu11112716
Park E, Kim J, Yeo S, Lim E, Choi CW, Choi S, Li WY, Lee J-W, Park J-H, Huh D, et al. Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice. Nutrients. 2019; 11(11):2716. https://doi.org/10.3390/nu11112716
Chicago/Turabian StylePark, Eunkuk, Jeonghyun Kim, Subin Yeo, Eunguk Lim, Chun Whan Choi, Sangho Choi, Wan Yi Li, Ji-Won Lee, Jin-Hyok Park, Dam Huh, and et al. 2019. "Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice" Nutrients 11, no. 11: 2716. https://doi.org/10.3390/nu11112716