Effect of Milk Fermented with Lactobacillus fermentum on the Inflammatory Response in Mice
AbstractCurrently, the effect of fermented milk on the T-helper 17 response in inflammatory bowel diseases (IBDs) is unknown. The aim of the present study was to evaluate the effect of milks fermented with Lactobacillus fermentum on the Th1/Th17 response in a murine model of mild IBD. Exopolysaccharide (EPS), lactic acid (LA), and total protein (TP) contents and bacterial concentration were determined. Male C57Bl/6 mice intragastrically received either raw (FM) or pasteurized (PFM) fermented milk before and during a dextran sulfate infusion protocol. Blood, spleen, and colon samples were collected at Weeks 6 and 10. IL-6, IL-10, and TNFα were determined in serum, and IL-17, IL-23, and IFNγ were determined in intestinal mucosa and serum. The FM groups did not differ in cell concentration, LA, or TP content (p > 0.05); FM-J28 had the highest EPS content. Spleen weight and colon length did not differ among the FM groups (p > 0.05). In the FM-J20 and PFM-J20 groups, IL-17 and IFNγ decreased, and the IL-10 concentration was enhanced (p < 0.05) at Week 6. IL-6, TNFα, IL-23, and IFNγ did not differ in serum and mucosa (p > 0.05), and IL-17 was lowest in FM-J28 and FM-J20. Therefore, FM appears to potentially play a role in decreasing the Th17 response. However, further studies are needed to elucidate the FM-mediated anti-inflammatory mechanisms in IBD. View Full-Text
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Santiago-López, L.; Hernández-Mendoza, A.; Mata-Haro, V.; Vallejo-Córdoba, B.; Wall-Medrano, A.; Astiazarán-García, H.; Estrada-Montoya, M.C.; González-Córdova, A.F. Effect of Milk Fermented with Lactobacillus fermentum on the Inflammatory Response in Mice. Nutrients 2018, 10, 1039.
Santiago-López L, Hernández-Mendoza A, Mata-Haro V, Vallejo-Córdoba B, Wall-Medrano A, Astiazarán-García H, Estrada-Montoya MC, González-Córdova AF. Effect of Milk Fermented with Lactobacillus fermentum on the Inflammatory Response in Mice. Nutrients. 2018; 10(8):1039.Chicago/Turabian Style
Santiago-López, Lourdes; Hernández-Mendoza, Adrián; Mata-Haro, Verónica; Vallejo-Córdoba, Belinda; Wall-Medrano, Abraham; Astiazarán-García, Humberto; Estrada-Montoya, María C.; González-Córdova, Aarón F. 2018. "Effect of Milk Fermented with Lactobacillus fermentum on the Inflammatory Response in Mice." Nutrients 10, no. 8: 1039.
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