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Volume 11
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10.3390/pharmaceutics11100514
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Article

Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets

by
Rana Bakhaidar
1,
Joshua Green
2,
Khaled Alfahad
2,
Shazia Samanani
2,
Nabeehah Moollan
2,
Sarah O’Neill
1 and
Zebunnissa Ramtoola
1,*
1
School of Pharmacy and Biological Sciences, Royal College of Surgeons in Ireland, 2 Dublin, Ireland
2
School of Medicine, Royal College of Surgeons in Ireland, 2 Dublin, Ireland
*
Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(10), 514; https://doi.org/10.3390/pharmaceutics11100514
Submission received: 23 August 2019 / Revised: 25 September 2019 / Accepted: 26 September 2019 / Published: 4 October 2019
(This article belongs to the Special Issue PLGA Based Drug Carrier and Pharmaceutical Applications)
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Abstract

Nanotechnology is being increasingly utilised in medicine as diagnostics and for drug delivery and targeting. The small size and high surface area of nanoparticles (NPs), desirable properties that allow them to cross biological barriers, also offer potential for interaction with other cells and blood constituents, presenting possible safety risks. While NPs investigated are predominantly based on the biodegradable, biocompatible, and FDA approved poly-lactide-co-glycolide (PLGA) polymers, pro-aggregatory and antiplatelet effects have been reported for certain NPs. The potential for toxicity of PLGA based NPs remains to be examined. The aims of this study were to determine the impact of size-selected PLGA-PEG (PLGA-polyethylene glycol) NPs on platelet activation and aggregation. PLGA-PEG NPs of three average sizes of 112, 348, and 576 nm were formulated and their effect at concentrations of 0.0–2.2 mg/mL on the activation and aggregation of washed human platelets (WP) was examined. The results of this study show, for the first time, NPs of all sizes associated with the surface of platelets, with >50% binding, leading to possible internalisation. The NP-platelet interaction, however, did not lead to platelet aggregation nor inhibited aggregation of platelets induced by thrombin. The outcome of this study is promising, suggesting that these NPs could be potential carriers for targeted drug delivery to platelets.
Keywords: PLGA-PEG; nanoparticles; platelet; activation; aggregation; binding; uptake PLGA-PEG; nanoparticles; platelet; activation; aggregation; binding; uptake
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MDPI and ACS Style

Bakhaidar, R.; Green, J.; Alfahad, K.; Samanani, S.; Moollan, N.; O’Neill, S.; Ramtoola, Z. Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets. Pharmaceutics 2019, 11, 514. https://doi.org/10.3390/pharmaceutics11100514

AMA Style

Bakhaidar R, Green J, Alfahad K, Samanani S, Moollan N, O’Neill S, Ramtoola Z. Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets. Pharmaceutics. 2019; 11(10):514. https://doi.org/10.3390/pharmaceutics11100514

Chicago/Turabian Style

Bakhaidar, Rana, Joshua Green, Khaled Alfahad, Shazia Samanani, Nabeehah Moollan, Sarah O’Neill, and Zebunnissa Ramtoola. 2019. "Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets" Pharmaceutics 11, no. 10: 514. https://doi.org/10.3390/pharmaceutics11100514

APA Style

Bakhaidar, R., Green, J., Alfahad, K., Samanani, S., Moollan, N., O’Neill, S., & Ramtoola, Z. (2019). Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets. Pharmaceutics, 11(10), 514. https://doi.org/10.3390/pharmaceutics11100514

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