The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
Abstract
:1. Introduction
2. Materials and Methods
2.1. PRRSV-2 Propagation and Titration
2.2. Study Design
2.3. Blood and Tissue Sample Processing
2.4. Immunotyping of Tissue Samples
2.5. Cytokine Production of PRRSV-Stimulated T-cell Subsets
2.6. Proliferation of PRRSV-Stimulated T-cell Subsets
2.7. Viremia
2.8. Statistical Analysis
3. Results
3.1. Clinical Measures, and Viremia
3.2. Time Kinetic of the Systemic Proliferative T-cell Response to Homologous Restimulation
3.3. CD4+ T-Cell Immune Response to Homo- and Heterologous PRRSV-2 Strains at 28 dpi
3.4. CD3+CD8α+ T-Cell (CTLs) Immune Response to Homo- and Heterologous PRRSV-2 Strains at 28 dpi
3.5. TCR-γδ T Cells Ex Vivo Immune Response to Heterologous PRRSV-2 Strains at 28 dpi
3.6. The T-cell Response to PRRSV-2 in the Tracheobronchial Lymph Node at 63 Days Post Infection
3.7. Distribution of Immune Cell Subsets and Prevalence of PRRSV-2 in the Lung and in Lymphoid Tissues at 63 dpi
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kick, A.R.; Amaral, A.F.; Cortes, L.M.; Fogle, J.E.; Crisci, E.; Almond, G.W.; Käser, T. The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Viruses 2019, 11, 796. https://doi.org/10.3390/v11090796
Kick AR, Amaral AF, Cortes LM, Fogle JE, Crisci E, Almond GW, Käser T. The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Viruses. 2019; 11(9):796. https://doi.org/10.3390/v11090796
Chicago/Turabian StyleKick, Andrew R., Amanda F. Amaral, Lizette M. Cortes, Jonathan E. Fogle, Elisa Crisci, Glen W. Almond, and Tobias Käser. 2019. "The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)" Viruses 11, no. 9: 796. https://doi.org/10.3390/v11090796