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Open AccessBrief Report

Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation

1
MIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, France
2
Unité des virus émergents, Aix Marseille Univ-IRD 190, Inserm 1207-IHU Méditerranée Infection, 13385 Marseille, France
3
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand
4
PhyMedExp, CNRS UMR 9214, INSERM U1046, University of Montpellier, 34295 Montpellier, France
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(9), 797; https://doi.org/10.3390/v11090797
Received: 9 July 2019 / Revised: 26 August 2019 / Accepted: 27 August 2019 / Published: 29 August 2019
(This article belongs to the Special Issue Emerging Arboviruses)
Mayaro virus (MAYV) is an emerging arthritogenic alphavirus belonging to the Togaviridae family. Infection leads to a dengue-like illness accompanied by severe polyarthralgia. However, the molecular and cellular mechanisms of arthritis as a result of MAYV infection remain poorly understood. In the present study, we assess the susceptibility of human chondrocytes (HC), fibroblast-like synoviocytes and osteoblasts that are the major cell types involved in osteoarthritis, to infection with MAYV. We show that these cells are highly permissive to MAYV infection and that viral RNA copy number and viral titers increase over time in infected cells. Knowing that HC are the primary cells in articular cartilage and are essential for maintaining the cartilaginous matrix, gene expression studies were conducted in MAYV-infected primary HC using polymerase chain reaction (PCR) arrays. The infection of the latter cells resulted in an induction in the expression of several matrix metalloproteinases (MMP) including MMP1, MMP7, MMP8, MMP10, MMP13, MMP14 and MMP15 which could be involved in the destruction of articular cartilage. Infected HC were also found to express significantly increased levels of various IFN-stimulated genes and arthritogenic mediators such as TNF-α and IL-6. In conclusion, MAYV-infected primary HC overexpress arthritis-related genes, which may contribute to joint degradation and pathogenesis. View Full-Text
Keywords: arbovirus; Mayaro virus; matrix metalloproteinases; extracellular matrix; chondrocyte; synoviocyte; osteoblast; innate immunity; IL-6; TNF-α arbovirus; Mayaro virus; matrix metalloproteinases; extracellular matrix; chondrocyte; synoviocyte; osteoblast; innate immunity; IL-6; TNF-α
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MDPI and ACS Style

Bengue, M.; Ferraris, P.; Baronti, C.; Diagne, C.T.; Talignani, L.; Wichit, S.; Liegeois, F.; Bisbal, C.; Nougairède, A.; Missé, D. Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation. Viruses 2019, 11, 797. https://doi.org/10.3390/v11090797

AMA Style

Bengue M, Ferraris P, Baronti C, Diagne CT, Talignani L, Wichit S, Liegeois F, Bisbal C, Nougairède A, Missé D. Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation. Viruses. 2019; 11(9):797. https://doi.org/10.3390/v11090797

Chicago/Turabian Style

Bengue, Michèle; Ferraris, Pauline; Baronti, Cécile; Diagne, Cheikh T.; Talignani, Loïc; Wichit, Sineewanlaya; Liegeois, Florian; Bisbal, Catherine; Nougairède, Antoine; Missé, Dorothée. 2019. "Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation" Viruses 11, no. 9: 797. https://doi.org/10.3390/v11090797

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