Identification, Classification and Expression Analysis of the CesA Gene Family from Pinus massoniana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Identification of CesA Genes in P. massoniana
2.3. Total RNA Extraction and Cloning ORF Sequences of -CesA Genes in P. massoniana
2.4. Sequence and Phylogenetic Analyses
2.5. Subcellular Localization
2.6. Real-Time Quantitative PCR
3. Results
3.1. Identification of CesA Genes and Cloning of Their ORF Sequences in P. massoniana
3.2. Structure Analysis of CESA Proteins in P. massoniana
3.3. Sequence and Phylogenetic Analyses of CesA Proteins in P. massoniana
3.4. Subcellular Localization of CesA Protein
3.5. Expression Levels of CesA Genes in Different Tissues or Organs
3.6. Expression Levels of CesA Genes under Different Treatments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene ID | ORF (bp) | Amino Acid No. | Molecular Mass (Da) | Isoelectric Points | Predicted TMHs 1 No. | Subcellular Localization |
---|---|---|---|---|---|---|
Transcript4609 | 2955 | 984 | 110807.39 | 5.96 | 6 | Plasma membrane |
Transcript2643 | 3174 | 1057 | 119729.90 | 8.27 | 8 | Plasma membrane |
Transcript1263 | 3255 | 1084 | 122204.09 | 6.70 | 8 | Plasma membrane |
Transcript691 | 3291 | 1096 | 123411.08 | 6.42 | 8 | Plasma membrane |
Transcript1283 | 3282 | 1093 | 123212.84 | 6.21 | 6 | Plasma membrane |
Transcript418 | 3306 | 1101 | 123447.25 | 6.60 | 8 | Plasma membrane |
Transcript556 | 3288 | 1095 | 123780.26 | 6.57 | 8 | Plasma membrane |
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Hou, Y.; Wang, D.; Agassin, R.H.; Zhang, C.; Lou, X.; Zhu, P.; Zhang, M.; Huang, Z.; Ji, K. Identification, Classification and Expression Analysis of the CesA Gene Family from Pinus massoniana. Forests 2023, 14, 1035. https://doi.org/10.3390/f14051035
Hou Y, Wang D, Agassin RH, Zhang C, Lou X, Zhu P, Zhang M, Huang Z, Ji K. Identification, Classification and Expression Analysis of the CesA Gene Family from Pinus massoniana. Forests. 2023; 14(5):1035. https://doi.org/10.3390/f14051035
Chicago/Turabian StyleHou, Yanqing, Dengbao Wang, Romaric Hippolyte Agassin, Chi Zhang, Xuan Lou, Peihuang Zhu, Mengyang Zhang, Zichen Huang, and Kongshu Ji. 2023. "Identification, Classification and Expression Analysis of the CesA Gene Family from Pinus massoniana" Forests 14, no. 5: 1035. https://doi.org/10.3390/f14051035