Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, Valencia 46022, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Valencia 46022, Spain
Materials Chemistry Laboratory, Department of Chemistry, Gulbarga University, Gulbarga, Karnataka 585106, India
Biological Research Innovation Centre and Solutions LLP, Bengaluru, Karnataka 56004, India
Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain
Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València, Camino de Vera 14, Valencia 46022, Spain
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Carlos Lodeiro
Received: 30 March 2016 / Revised: 21 April 2016 / Accepted: 25 April 2016 / Published: 28 April 2016
Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1
], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both the environmental and biomedical applications, due to their eco-friendly nature and the avoidance of toxic chemicals in the synthesis. For this reason, employing bio routes in the synthesis of functionalized silver nanoparticles (FAgNP) have gained importance recently in this field. In the present study, we report the rapid synthesis of FAgNP through the extract of pepino (Solanum muricatum
) leaves and employing microwave oven irradiation. The core-shell globular morphology and characterization of the different shaped and sized FAgNP, with a core of 20–50 nm of diameter is established using the UV-Visible spectroscopy (UV-vis), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Zeta potential and dynamic light scanning (DLS) studies. Moreover, cytotoxic studies employing HeLa (human cervix carcinoma) cells were undertaken to understand FAgNP interactions with cells. HeLa cells showed significant dose dependent antiproliferative activity in the presence of FAgNP at relatively low concentrations. The calculated IC50
value was 37.5 µg/mL, similar to others obtained for FAgNPs against HeLa cells.
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Gorbe, M.; Bhat, R.; Aznar, E.; Sancenón, F.; Marcos, M.D.; Herraiz, F.J.; Prohens, J.; Venkataraman, A.; Martínez-Máñez, R. Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells. Materials 2016, 9, 325.
Gorbe M, Bhat R, Aznar E, Sancenón F, Marcos MD, Herraiz FJ, Prohens J, Venkataraman A, Martínez-Máñez R. Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells. Materials. 2016; 9(5):325.
Gorbe, Mónica; Bhat, Ravishankar; Aznar, Elena; Sancenón, Félix; Marcos, M. D.; Herraiz, F. J.; Prohens, Jaime; Venkataraman, Abbaraju; Martínez-Máñez, Ramón. 2016. "Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells." Materials 9, no. 5: 325.
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