Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells

Ros-Tárraga, Patricia; Rabadan-Ros, Rubén; Murciano, Angel; Meseguer-Olmo, Luis; De Aza, Piedad N.

doi:10.3390/ma9120969

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Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells

¹,

¹,

²,

and

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Grupo de Investigación en Regeneración y Reparación de Tejidos, UCAM—Universidad Católica San Antonio de Murcia, Guadalupe, 30107 Murcia, Spain

Departamento de Materiales, Óptica y Tecnologia Electrónica, Universidad Miguel Hernández, Avda. Universidad s/n, 03202 Elche (Alicante), Spain

Service of Orthopaedic at Arrixaca University Hospital, UCAM—Catholic University of Murcia, 30120 Murcia, Spain

⁴

Instituto de Bioingenieria, Universidad Miguel Hernandez, Avda. Ferrocarril s/n. Elche, 03202 Alicante, Spain

Author to whom correspondence should be addressed.

Academic Editor: Enrico Bernardo

Materials 2016, 9(12), 969; https://doi.org/10.3390/ma9120969

Received: 12 October 2016 / Revised: 11 November 2016 / Accepted: 15 November 2016 / Published: 29 November 2016

(This article belongs to the Special Issue Bioceramics 2016)

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Abstract

A new type of bioceramic with osteogenic properties, suitable for hard tissue regeneration, was synthesised. The ceramic was designed and obtained in the Nurse’s A-phase-silicocarnotite subsystem. The selected composition was that corresponding to the eutectoid 28.39 wt % Nurse’s A-phase-71.61 wt % silicocarnotite invariant point. We report the effect of Nurse’s A-phase-silicocarnotite ceramic on the capacity of multipotent adult human mesenchymal stem cells (ahMSCs) cultured under experimental conditions, known to adhere, proliferate and differentiate into osteoblast lineage cells. The results at long-term culture (28 days) on the material confirmed that the undifferentiated ahMSCs cultured and in contact with the material surface adhered, spread, proliferated, and produced a mineralised extracellular matrix on the studied ceramic, and finally acquired an osteoblastic phenotype. These findings indicate that it underwent an osteoblast differentiation process. All these findings were more significant than when cells were grown on plastic, in the presence and absence of this osteogenic supplement, and were more evident when this supplement was present in the growth medium (GM). The ceramic evaluated herein was bioactive, cytocompatible and capable of promoting the proliferation and differentiation of undifferentiated ahMSCs into osteoblasts, which may be important for bone integration into the clinical setting. View Full-Text

Keywords: bioceramic; calcium silicophosphate; eutectoid; adult human mesenchymal stem cells; cell culture; osteogenic differentiation

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MDPI and ACS Style

Ros-Tárraga, P.; Rabadan-Ros, R.; Murciano, A.; Meseguer-Olmo, L.; De Aza, P.N. Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells. Materials 2016, 9, 969. https://doi.org/10.3390/ma9120969

AMA Style

Ros-Tárraga P, Rabadan-Ros R, Murciano A, Meseguer-Olmo L, De Aza PN. Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells. Materials. 2016; 9(12):969. https://doi.org/10.3390/ma9120969

Chicago/Turabian Style

Ros-Tárraga, Patricia, Rubén Rabadan-Ros, Angel Murciano, Luis Meseguer-Olmo, and Piedad N. De Aza. 2016. "Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells" Materials 9, no. 12: 969. https://doi.org/10.3390/ma9120969

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Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells

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