Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
Department of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, Brazil
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Materials 2020, 13(7), 1751; https://doi.org/10.3390/ma13071751
Received: 11 March 2020
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Revised: 27 March 2020
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Accepted: 7 April 2020
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Published: 10 April 2020
(This article belongs to the Special Issue Advanced Functional Nanostructured Biosensors)
A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L−1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.
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MDPI and ACS Style
Rocha, G.S.; Silva, M.K.L.; Cesarino, I. Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples. Materials 2020, 13, 1751. https://doi.org/10.3390/ma13071751
AMA Style
Rocha GS, Silva MKL, Cesarino I. Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples. Materials. 2020; 13(7):1751. https://doi.org/10.3390/ma13071751
Chicago/Turabian StyleRocha, Giovanna S., Martin K.L. Silva, and Ivana Cesarino. 2020. "Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples" Materials 13, no. 7: 1751. https://doi.org/10.3390/ma13071751
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