Next Article in Journal
Modelling Inhomogeneity of Veneer Laminates with a Finite Element Mapping Method Based on Arbitrary Grayscale Images
Next Article in Special Issue
Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER®
Previous Article in Journal
Enhanced Osteogenic Differentiation of Human Primary Mesenchymal Stem and Progenitor Cultures on Graphene Oxide/Poly(methyl methacrylate) Composite Scaffolds
Previous Article in Special Issue
Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
Article

Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

1
Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic
2
Department of Physical and Applied Chemistry, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic
3
Centre of Polymer Systems, Tomas Bata University in Zlin, trida Tomase Bati 5678, 760 01 Zlin, Czech Republic
*
Author to whom correspondence should be addressed.
Materials 2020, 13(13), 2992; https://doi.org/10.3390/ma13132992
Received: 11 June 2020 / Revised: 2 July 2020 / Accepted: 3 July 2020 / Published: 5 July 2020
(This article belongs to the Special Issue Biocompatible and Biodegradable 3D Scaffolds)
Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized by employing Cupriavidus necator grown on fructose (PHBV-1) or grape sugar extract (PHBV-2). Fifty grams of lyophilized grape sugar extract contained 19.2 g of glucose, 19.1 g of fructose, 2.7 g of pectin, 0.52 g of polyphenols, 0.51 g of flavonoids and 7.97 g of non-identified rest compounds. The grape sugar extract supported the higher production of biomass and modified the composition of PHBV-2. The biosynthesized PHAs served as matrices for the preparation of the scaffolds. The PHBV-2 scaffolds had about 44.2% lower crystallinity compared to the PHBV-1 scaffolds. The degree of crystallinity markedly influenced the mechanical behavior and enzymatic hydrolysis of the PHA scaffolds in the synthetic gastric juice and phosphate buffer saline solution with the lipase for 81 days. The higher proportion of amorphous moieties in PHBV-2 accelerated enzymatic hydrolysis. After 81-days of lasting enzymatic hydrolysis, the morphological changes of the PHBV-1 scaffolds were negligible compared to the visible destruction of the PHBV-2 scaffolds. These results indicated that the presence of pectin and phenolic moieties in PHBV may markedly change the semi-crystalline character of PHBV, as well as its mechanical properties and the course of abiotic or enzymatic hydrolysis. View Full-Text
Keywords: degradation kinetics; grape pomace; hydrolysis; mechanical properties; morphology; scaffolds; polyhydroxyalkanoates; thermal properties degradation kinetics; grape pomace; hydrolysis; mechanical properties; morphology; scaffolds; polyhydroxyalkanoates; thermal properties
Show Figures

Graphical abstract

MDPI and ACS Style

Kovalcik, A.; Obruca, S.; Kalina, M.; Machovsky, M.; Enev, V.; Jakesova, M.; Sobkova, M.; Marova, I. Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds. Materials 2020, 13, 2992. https://doi.org/10.3390/ma13132992

AMA Style

Kovalcik A, Obruca S, Kalina M, Machovsky M, Enev V, Jakesova M, Sobkova M, Marova I. Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds. Materials. 2020; 13(13):2992. https://doi.org/10.3390/ma13132992

Chicago/Turabian Style

Kovalcik, Adriana, Stanislav Obruca, Michal Kalina, Michal Machovsky, Vojtech Enev, Michaela Jakesova, Marketa Sobkova, and Ivana Marova. 2020. "Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds" Materials 13, no. 13: 2992. https://doi.org/10.3390/ma13132992

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop