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Open AccessArticle

Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts

1
CEITEC—Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00 Brno, Czech Republic
2
Tissue Bioengineering Laboratory, Faculty of Dentistry, Universidad Nacional Autónoma de México, Coyoacan, Mexico City 04510, Mexico
*
Author to whom correspondence should be addressed.
Materials 2020, 13(8), 1966; https://doi.org/10.3390/ma13081966
Received: 24 March 2020 / Revised: 16 April 2020 / Accepted: 20 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Biocompatible and Biodegradable 3D Scaffolds)
Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cryopreservation on biofabricated osteoblast-containing structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast post-thawing metabolic activity and the recovery of the osteoblastic function of 3D-bioprinted osteoblastic structures and beta tricalcium phosphate (β-TCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at −80 °C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The pre-cryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longer-term culture (7 days). The use of β-TCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the non-preserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice. View Full-Text
Keywords: biofabrication; bioprinting; cryopreservation; bone construct; osteoblast; metabolic activity; ALP activity biofabrication; bioprinting; cryopreservation; bone construct; osteoblast; metabolic activity; ALP activity
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MDPI and ACS Style

Hernández-Tapia, L.G.; Fohlerová, Z.; Žídek, J.; Alvarez-Perez, M.A.; Čelko, L.; Kaiser, J.; Montufar, E.B. Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts. Materials 2020, 13, 1966. https://doi.org/10.3390/ma13081966

AMA Style

Hernández-Tapia LG, Fohlerová Z, Žídek J, Alvarez-Perez MA, Čelko L, Kaiser J, Montufar EB. Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts. Materials. 2020; 13(8):1966. https://doi.org/10.3390/ma13081966

Chicago/Turabian Style

Hernández-Tapia, Laura G.; Fohlerová, Zdenka; Žídek, Jan; Alvarez-Perez, Marco A.; Čelko, Ladislav; Kaiser, Jozef; Montufar, Edgar B. 2020. "Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts" Materials 13, no. 8: 1966. https://doi.org/10.3390/ma13081966

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