Next Article in Journal
Environmental Screening of Electrode Materials for a Rechargeable Aluminum Battery with an AlCl3/EMIMCl Electrolyte
Next Article in Special Issue
A Novel Multiscale Mathematical Model for Building Bone Substitute Materials for Children
Previous Article in Journal
Corrosion Performance of Nano-ZrO2 Modified Coatings in Hot Mixed Acid Solutions
Previous Article in Special Issue
Histological Evaluation of the Healing Process of Various Bone Graft Materials after Engraftment into the Human Body
Open AccessArticle

Influence of Absorbable Calcium Sulfate-Based Bone Substitute Materials on Human Haemostasis—In Vitro Biological Behavior of Antibiotic Loaded Implants

1
Klinikum rechts der Isar der Technischen Universität München, Klinik und Poliklinik für Unfallchirurgie, Ismaninger Str. 22, 81675 München, Germany
2
Klinikum rechts der Isar der Technischen Universität München, Klinik für Orthopädie und Sportorthopädie, Ismaninger Str. 22, 81675 München, Germany
3
Chirurgisches Klinikum München Süd, Am Isarkanal 30, 81379 München, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors also contributed equally to this work.
Materials 2018, 11(6), 935; https://doi.org/10.3390/ma11060935
Received: 20 April 2018 / Revised: 16 May 2018 / Accepted: 30 May 2018 / Published: 1 June 2018
(This article belongs to the Special Issue Bone Substitute Materials)
Calcium sulfate (CS) formulations are frequently implanted as antibiotically impregnated bone substitutes in orthopedic and trauma surgery to prevent or treat bone infections. Calcium ions have been discussed as candidates to accelerate blood coagulation. The goal of this study is to evaluate substance-specific influences of CS formulations on blood coagulation. Specific ELISAs were conducted to determine markers of activated blood coagulation after incubation of human blood with CS beads. Additionally, wettability with freshly drawn human blood was measured. Three different types of CS bone substitute beads were compared (CS dihydrate with tripalmitin, containing Gentamicin (Herafill®-G: Group A) or Vancomycin (CaSO4-V: Group B); and a CS hemihydrate with Tobramycin (Osteoset®: Group C)). Examinations were performed by ELISA assays for F1+2, FXIIa and C3a. Our results prove that none of the CS preparations accelerated single specific assays for activated coagulation markers. This allows the conclusion that neither Herafill®-G (CaSO4-G) nor CaSO4-V alter haemostasis negatively. Blood samples incubated with Osteoset® display an elevated F1+2-activity. The addition of tripalmitin in Herafill®-G shifts the original into a significantly hydrophobic formulation. This was additionally proven by contact angle examination of the three substances with freshly drawn human blood, showing that acceleration of plasmatic coagulation is hindered by lipids and induced by surface effects caused by presence of rapidly soluble calcium ions in the Osteoset® preparation. View Full-Text
Keywords: calcium sulfate formulations; calcium carbonate; tripalmitin; coagulation; in vitro; Herafill®-G; Osteoset®, gentamicin; vancomycin; tobramycin; FXIIa; C3a; F1+2 calcium sulfate formulations; calcium carbonate; tripalmitin; coagulation; in vitro; Herafill®-G; Osteoset®, gentamicin; vancomycin; tobramycin; FXIIa; C3a; F1+2
Show Figures

Figure 1

MDPI and ACS Style

Pförringer, D.; Harrasser, N.; Beirer, M.; Crönlein, M.; Stemberger, A.; Van Griensven, M.; Lucke, M.; Burgkart, R.; Obermeier, A. Influence of Absorbable Calcium Sulfate-Based Bone Substitute Materials on Human Haemostasis—In Vitro Biological Behavior of Antibiotic Loaded Implants. Materials 2018, 11, 935.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop