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Article

Candida tropicalis as a Promising Oleaginous Yeast for Olive Mill Wastewater Bioconversion

Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Academic Editors: Diego Luna and Antonio Zuorro
Energies 2021, 14(3), 640; https://doi.org/10.3390/en14030640
Received: 23 December 2020 / Revised: 20 January 2021 / Accepted: 22 January 2021 / Published: 27 January 2021
(This article belongs to the Special Issue Biorefinery Based on Waste Biomass)
Olive mill wastewater (OMW), which is generated during olive oil production, has detrimental effects on the environment due to its high organic load and phenolic compounds content. OMW is difficult to biodegrade, but represents a valuable resource of nutrients for microbial growth. In this study, yeast strains were screened for their growth on phenolic compounds usually found in OMW and responsible for antimicrobial effects. Candida tropicalis ATCC 750 demonstrated an extraordinary capacity to grow in phenolics and was chosen for further experiments with OMW-based medium. The effects of nitrogen supplementation, the pH, and the stirring rate on cellular growth, OMW-components consumption, and added-value compounds production were studied in batch cultures in Erlenmeyer flasks and in a bioreactor. Candida tropicalis was able to reduce 68% of the organic load (chemical oxygen demand) and 39% of the total phenols of OMW in optimized conditions in bioreactor experiments, producing lipase (203 U·L−1) and protease (1105 U·L−1). Moreover, intracellular lipids were accumulated, most significantly under nitrogen-limited conditions, which is common in this type of wastewater. The high potential of C. tropicalis to detoxify OMW and produce added-value compounds from it makes this process an alternative approach to other conventional processes of OMW treatment. View Full-Text
Keywords: olive mill wastewater; phenolic compounds; Candida tropicalis; microbial lipids; lipase; protease olive mill wastewater; phenolic compounds; Candida tropicalis; microbial lipids; lipase; protease
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MDPI and ACS Style

Dias, B.; Lopes, M.; Ramôa, R.; Pereira, A.S.; Belo, I. Candida tropicalis as a Promising Oleaginous Yeast for Olive Mill Wastewater Bioconversion. Energies 2021, 14, 640. https://doi.org/10.3390/en14030640

AMA Style

Dias B, Lopes M, Ramôa R, Pereira AS, Belo I. Candida tropicalis as a Promising Oleaginous Yeast for Olive Mill Wastewater Bioconversion. Energies. 2021; 14(3):640. https://doi.org/10.3390/en14030640

Chicago/Turabian Style

Dias, Bruna, Marlene Lopes, Renata Ramôa, Ana S. Pereira, and Isabel Belo. 2021. "Candida tropicalis as a Promising Oleaginous Yeast for Olive Mill Wastewater Bioconversion" Energies 14, no. 3: 640. https://doi.org/10.3390/en14030640

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