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Mar. Drugs 2018, 16(11), 447; https://doi.org/10.3390/md16110447

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

1
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
2
Department of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, Italy
*
Authors to whom correspondence should be addressed.
Received: 30 September 2018 / Revised: 10 November 2018 / Accepted: 12 November 2018 / Published: 15 November 2018
(This article belongs to the Special Issue Marine Chitin 2019)
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Abstract

Most of the methods of poly-lactic-glycolic acid (PLGA) preparation involve the passage through the emulsification of a PLGA organic solution in water followed by solvent evaporation or extraction. The choice of the droplet stabilizer during the emulsion step is critical for the dimensions and the surface characteristics of the nanoparticles (NPs). In the present work, a recently described ionic amphiphilic chitosan derivative, chitosan oleate salt (CS-OA), was proposed for the first time to prepare PLGA NPs. A full factorial design was used to understand the effect of some formulation and preparation parameters on the NP dimensions and on encapsulation efficiency (EE%) of Nile red, used as a tracer. On the basis of the DoE study, curcumin loaded NPs were prepared, having 329 ± 42 nm dimensions and 68.75% EE%. The presence of a chitosan coating at the surface was confirmed by positive zeta potential and resulted in mucoadhesion behavior. The expected improvement of the interaction of the chitosan surface modified nanoparticles with cell membrane surface was confirmed in Caco-2 cell culture by the internalization of the loaded curcumin. View Full-Text
Keywords: chitosan oleate salt; amphiphilic polymer; PLGA; nanoparticles; mucoadhesion; Caco-2 cell culture; nile red; curcumin chitosan oleate salt; amphiphilic polymer; PLGA; nanoparticles; mucoadhesion; Caco-2 cell culture; nile red; curcumin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Miele, D.; Rossi, S.; Sandri, G.; Vigani, B.; Sorrenti, M.; Giunchedi, P.; Ferrari, F.; Bonferoni, M.C. Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties. Mar. Drugs 2018, 16, 447.

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