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Article

Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides

by
Chandra Sekhar Bokka
1,
Ganesh Kumar Veeramachaneni
1,
V. B. S. C. Thunuguntla
1,
Janakiram Bobbillapati
2 and
Jayakumar Singh Bondili
1,*
1
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Green fields, Vaddeswaram, Guntur Dist., Andhra Pradesh 522 502, India
2
Department of Microbiology, NRI Medical college and General Hospital, Guntur Dist., Andhra Pradesh 522 503, India
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(5), 178; https://doi.org/10.3390/medicina55050178
Submission received: 30 January 2019 / Revised: 16 March 2019 / Accepted: 17 May 2019 / Published: 21 May 2019
(This article belongs to the Special Issue Pollen Allergies)
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Abstract

Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (−9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
Keywords: Sorghum; pollen allergen; profilin; peptide mapping; modeling; Th1 and Th2 cytokine Sorghum; pollen allergen; profilin; peptide mapping; modeling; Th1 and Th2 cytokine
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MDPI and ACS Style

Bokka, C.S.; Veeramachaneni, G.K.; Thunuguntla, V.B.S.C.; Bobbillapati, J.; Bondili, J.S. Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides. Medicina 2019, 55, 178. https://doi.org/10.3390/medicina55050178

AMA Style

Bokka CS, Veeramachaneni GK, Thunuguntla VBSC, Bobbillapati J, Bondili JS. Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides. Medicina. 2019; 55(5):178. https://doi.org/10.3390/medicina55050178

Chicago/Turabian Style

Bokka, Chandra Sekhar, Ganesh Kumar Veeramachaneni, V. B. S. C. Thunuguntla, Janakiram Bobbillapati, and Jayakumar Singh Bondili. 2019. "Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides" Medicina 55, no. 5: 178. https://doi.org/10.3390/medicina55050178

APA Style

Bokka, C. S., Veeramachaneni, G. K., Thunuguntla, V. B. S. C., Bobbillapati, J., & Bondili, J. S. (2019). Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides. Medicina, 55(5), 178. https://doi.org/10.3390/medicina55050178

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