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Article

Analysis of Endoplasmic Reticulum Stress Proteins in Spermatogenic Cells After Paclitaxel Administration

by
Suna Karadeniz Saygılı
1,*,
Meryem Cansu Sahin
2,
Fulya Yukcu
3 and
Senem Sanli
2
1
Department of Histology and Embriyology, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43100, Türkiye
2
Department of Medical Services and Techniques, Vocational School of Health Services, Usak University, Usak 64100, Türkiye
3
Department of Biophysics, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43100, Türkiye
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2025, 47(8), 620; https://doi.org/10.3390/cimb47080620
Submission received: 7 July 2025 / Revised: 2 August 2025 / Accepted: 2 August 2025 / Published: 5 August 2025
(This article belongs to the Special Issue Pharmacological Activities and Mechanisms of Action of Natural Products)
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Abstract

Background/Objectives: The aim of this research is to analyze the effect of paclitaxel on endoplasmic reticulum (ER) stress in spermatogenic cells. Methods: In the study, spermatogonium (GC1) and spermatocyte (GC2) cell lines were used. The IC50 dose of paclitaxel was calculated using an MTT assay. Each cell line was separated into two different groups: control (GC1-C, GC2-C) and paclitaxel-treated (GC1-P, GC2-P). The control cells were incubated under standard culture conditions. The paclitaxel group cells were incubated in culture medium containing the paclitaxel IC50 dose for 24 h. After the experiments, all groups were stained with GRP78, p-PERK, and p-eIF2α antibodies using semi-quantitative immunocytochemistry. Results: Paclitaxel showed cytotoxicity. In the experimental model of the paclitaxel-treated cells, all the markers showed elevated levels of immunoreactivity, indicating ER stress. Conclusions: Paclitaxel administration triggered ER stress in spermatogenic cells. Studies of ER-related stress mechanisms in spermatogenic cells with further advanced molecular analyses will be important for therapeutic strategies.
Keywords: spermatogenesis; GC1; GC2; cell culture; endoplasmic reticulum; paclitaxel spermatogenesis; GC1; GC2; cell culture; endoplasmic reticulum; paclitaxel

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MDPI and ACS Style

Karadeniz Saygılı, S.; Sahin, M.C.; Yukcu, F.; Sanli, S. Analysis of Endoplasmic Reticulum Stress Proteins in Spermatogenic Cells After Paclitaxel Administration. Curr. Issues Mol. Biol. 2025, 47, 620. https://doi.org/10.3390/cimb47080620

AMA Style

Karadeniz Saygılı S, Sahin MC, Yukcu F, Sanli S. Analysis of Endoplasmic Reticulum Stress Proteins in Spermatogenic Cells After Paclitaxel Administration. Current Issues in Molecular Biology. 2025; 47(8):620. https://doi.org/10.3390/cimb47080620

Chicago/Turabian Style

Karadeniz Saygılı, Suna, Meryem Cansu Sahin, Fulya Yukcu, and Senem Sanli. 2025. "Analysis of Endoplasmic Reticulum Stress Proteins in Spermatogenic Cells After Paclitaxel Administration" Current Issues in Molecular Biology 47, no. 8: 620. https://doi.org/10.3390/cimb47080620

APA Style

Karadeniz Saygılı, S., Sahin, M. C., Yukcu, F., & Sanli, S. (2025). Analysis of Endoplasmic Reticulum Stress Proteins in Spermatogenic Cells After Paclitaxel Administration. Current Issues in Molecular Biology, 47(8), 620. https://doi.org/10.3390/cimb47080620

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