Probiotic Formulations Containing Fixed and Essential Oils Ameliorates SIBO-Induced Gut Dysbiosis in Rats
, Leyla Tarhan Celebi 2,3, Hulya Kayhan 4, Emine Kizilay 5, Mustafa Yavuz Gulbahar 6, Halil Kurt 7 and Bekir Cakici 2,3
Round 1
Reviewer 1 Report
Comments
The present study reported that the therapeutic effect of probiotic form containing Coconut oil and trace amounts of Peppermint-Lemon-Patchouli essential oil on SIBO. Study is interesting however, below mentioned points as well native person needs to polish English version.
Line 19-22: Rephrase the sentence “There is increasing evidence that dysbiosis of the gut microbiota is associated with the pathogenesis of intestinal diseases such as inflammatory bowel disease, irritable bowel syndrome (IBS) and small intestinal bacterial overgrowth (SIBO), and metabolic disease states such as allergies, cardiovascular diseases, and obesity and diabetes”.
Line 38: In keywords what is the abbreviation of SIBO?
Line 84-86: However, there are only a few studies in the literature reporting the efficacy of antibiotic treatment of bacterial overgrowth and antibiotics are insufficient for SIBO decontamination, alone rephrase the sentence and add the recent references.
Line 88: Add sentence “Probiotics are effective and appealing approach in the prevention of different diseases” and add the recent references such as https://doi.org/10.1080/00439339.2021.1883412 and 10.29261/pakvetj/2021.00
Line 122-149: Clearly mentioned the number of experimental animal groups with their replication
Line 151: Check the picture clearly following journal format
Line 194-195: Check the table clearly following journal format and some words are italic correct them
Line 451: Here S. boulardii should be italic
Line 454: Here S. Boulardii should be italic
Line 444: Wagner et al. examined the effects of probiotic supplementation on gastrointestinal symptoms and SIBO after “Gastric Bypass” here correct the reference following journal standard format
Line 447: Here B.lactis should be italic and check all over the manuscript
Line 500-502: What the future perspectives researchers can take following your trial? need more details
Comment: Need to check and improve figures, diagrams, and references following the journal format
Native person needs to polish English version.
Author Response
Reviewer 1
The present study reported that the therapeutic effect of probiotic form containing Coconut oil and trace amounts of Peppermint-Lemon-Patchouli essential oil on SIBO.
- Study is interesting however, below mentioned points as well native person needs to polish English version.
Response: Firstly, I really thank you so much for your supportive revisions. The manuscript was read by a native researcher.
- Line 19-22: Rephrase the sentence “There is increasing evidence that dysbiosis of the gut microbiota is associated with the pathogenesis of intestinal diseases such as inflammatory bowel disease, irritable bowel syndrome (IBS) and small intestinal bacterial overgrowth (SIBO), and metabolic disease states such as allergies, cardiovascular diseases, and obesity and diabetes”.
Response: It was revised according to the reviewer’s suggestion.
- Line 38: In keywords what is the abbreviation of SIBO?
Response: It was revised according to the reviewer’s suggestion.
- Line 84-86: However, there are only a few studies in the literature reporting the efficacy of antibiotic treatment of bacterial overgrowth and antibiotics are insufficient for SIBO decontamination, alone rephrase the sentence and add the recent references.
Response: It was revised according to the reviewer’s suggestion.
- Line 88: Add sentence “Probiotics are effective and appealing approach in the prevention of different diseases” and add the recent references such as https://doi.org/10.1080/00439339.2021.1883412 and 10.29261/pakvetj/2021.00
Response: The sentence was added according to the reviewer’s suggestion.
- Line 122-149: Clearly mentioned the number of experimental animal groups with their replication
Response: It was revised according to the reviewer’s suggestion.
- Line 151: Check the picture clearly following journal format
Response: It was revised according to the journal format.
- Line 194-195: Check the table clearly following journal format and some words are italic correct them
Response: It was revised according to the journal format.
- Line 451: Here S. boulardii should be italic
Response: It was corrected.
- Line 454: Here S. Boulardii should be italic
Response: It was corrected.
- Line 444: Wagner et al. examined the effects of probiotic supplementation on gastrointestinal symptoms and SIBO after “Gastric Bypass” here correct the reference following journal standard format
Response: It was revised according to the journal format.
- Line 447: Here B.lactis should be italic and check all over the manuscript
Response: It was corrected.
- Line 500-502: What the future perspectives researchers can take following your trial? need more details
Response: A sentence was added according to the reviewer’s suggestion.
- Comment: Need to check and improve figures, diagrams, and references following the journal format
Response: All checked according to the journal format.
Reviewer 2 Report
The authors aimed to evaluate the symbiotic (synergistic) effect of a probiotic mix (P+EO; Table 2, 9.2 x 109 CFU.mL-1) + essential oil mix [coconut (98.5%)/peppermint-lemon-patchouli (1.5%)] in rats with antibiotic/polyacrylamide/trans-fat-induced enteral dysbiosis [small intestinal bacterial overgrowth (SIBO)]. The anti-inflammatory potential (Histology/interleukins) and microbiota features (16S rRNA Meta sequencing). Differences in beta diversity (PERMNOVA, p≤0.024) and genus level (LEfSe analysis) were detected among groups and anti-inflammatory features where group-specific in a healthy>P+EO>control groups (P, EO alone)>untreated rats’ trend. Minor changes could further improve the study´s scientific soundness:
Title. Very long and with abbreviations. Suggestion: A probiotic consortium + essential oils, ameliorates antibiotic-induced gut dysbiosis in rats.
Abstract. Please describe more quantitatively than qualitatively (include p-values)
Introduction/methods. OK
Tables & Figures. A) All tables and figures (increase resolution >300 dpi) should be supplied according to the journal´s guidelines. B) Please consider providing them some of them as supplementary material. C) Figure 4: chloroplast? mitochondria? D) “The most common visible bacteria” are not visible, E) Significant figures (p-values) should not have more than three decimal places. F) Table 5 should describe significant differences. G) Provide enough details as footnotes when needed.
Discussion. Even though the probiotic + essential oil formulation used in this study is unique, the authors should make a more comparative discussion of the results obtained compared to other reports using a similar dysbiosis model and where symbiotic formulations are used to counteract it.
References. A) Review again the citation format according to the instructions for the authors. B) Reduce the ratio of new/old references (<10y) to say 25%.
Extensive editing of English language required
Author Response
Reviewer 2
The authors aimed to evaluate the symbiotic (synergistic) effect of a probiotic mix (P+EO; Table 2, 9.2 x 109 CFU.mL-1) + essential oil mix [coconut (98.5%)/peppermint-lemon-patchouli (1.5%)] in rats with antibiotic/polyacrylamide/trans-fat-induced enteral dysbiosis [small intestinal bacterial overgrowth (SIBO)]. The anti-inflammatory potential (Histology/interleukins) and microbiota features (16S rRNA Meta sequencing). Differences in beta diversity (PERMNOVA, p≤0.024) and genus level (LEfSe analysis) were detected among groups and anti-inflammatory features where group-specific in a healthy>P+EO>control groups (P, EO alone)>untreated rats’ trend. Minor changes could further improve the study´s scientific soundness:
- Title. Very long and with abbreviations. Suggestion: A probiotic consortium + essential oils, ameliorates antibiotic-induced gut dysbiosis in rats.
Response: Firstly, I really thank you so much for your supportive revisions. The title was revised according to the reviewer’s suggestion.
- Abstract. Please describe more quantitatively than qualitatively (include p-values)
Response: It was revised according to the reviewer’s suggestion.
- A) All tables and figures (increase resolution >300 dpi) should be supplied according to the journal´s guidelines.
Response: It was revised according to the journal’s format.
- B) Please consider providing them some of them as supplementary material.
Response: Thank the reviewer for his/her suggestion.
- C) Figure 4: chloroplast? mitochondria?
Response: It was revised according to the reviewer’s suggestion.
- D) “The most common visible bacteria” are not visible,
Response: It was revised according to the reviewer’s suggestion.
- E) Significant figures (p-values) should not have more than three decimal places.
Response: It was revised according to the reviewer’s suggestion.
- F) Table 5 should describe significant differences.
Response: The differences in significance were given in the results part.
- G) Provide enough details as footnotes when needed.
Response: It was revised according to the reviewer’s suggestion.
- Discussion. Even though the probiotic + essential oil formulation used in this study is unique, the authors should make a more comparative discussion of the results obtained compared to other reports using a similar dysbiosis model and where symbiotic formulations are used to counteract it.
References. A) Review again the citation format according to the instructions for the authors. B) Reduce the ratio of new/old references (<10y) to say 25%.
Reviewer 3 Report
I reviewed the manuscript titled “The Therapeutic Effect of Probiotic Form Containing Coconut Oil and Trace Amounts of Peppermint-Lemon-Patchouli Essential Oil on SIBO.
Please remove the colon (: punctuation mark) at the end of the title
Line 29: authors can remove the full name as it is already abbreviated elsewhere in the manuscript
Conclusions and recommendations of the study must be introduced in abstract
Introduction
Research gaps should be addressed with more recent literature. Please add more recent literature
Figure 1 quality must be improved. The test is, as such, is not readable
Scientific names must be in Italics
The section results are missing
Authors should keep the word
Most of the figures quality must be improved
The results presented are appropriate
The discussion section must be improved. As such, it is weak. I suggest authors to add more in-depth discussion on research findings and its comparison with available literature
References must be aligned with journal format
Author Response
Reviewer 3
I reviewed the manuscript titled “The Therapeutic Effect of Probiotic Form Containing Coconut Oil and Trace Amounts of Peppermint-Lemon-Patchouli Essential Oil on SIBO.
- Please remove the colon (: punctuation mark) at the end of the title
Response: Firstly, I really thank you so much for your supportive revisions. It was corrected.
- Line 29: authors can remove the full name as it is already abbreviated elsewhere in the manuscript
Response: It was revised according to the reviewer’s suggestion.
- Conclusions and recommendations of the study must be introduced in abstract
Response: It was revised according to the reviewer’s suggestion.
- Research gaps should be addressed with more recent literature. Please add more recent literature
Response: It was revised according to the reviewer’s suggestion.
- Figure 1 quality must be improved. The test is, as such, is not readable
Response: The figures were revised according to the reviewer’s suggestion.
- Scientific names must be in Italics
Response: It was revised according to the reviewer’s suggestion.
- The section results are missing
Response: It was revised according to the reviewer’s suggestion.
- Most of the figures quality must be improved
Response: The figures were revised according to the reviewer’s suggestion.
- The discussion section must be improved. As such, it is weak. I suggest authors to add more in-depth discussion on research findings and its comparison with the available literature
Response: The figures were revised according to the reviewer’s suggestion.
- References must be aligned with journal format
Response: It was revised according to the journal’s format.
Round 2
Reviewer 1 Report
Check the references carefully
Minor English editing is required
Reviewer 2 Report
Thanks for having accepted most of my suggestions
minor changes
