Synthesis, Physicochemical Characterization, Biological Evaluation, In Silico and Molecular Docking Studies of Pd(II) Complexes with P, S-Donor Ligands
, Amin Badshah 2, Sajjad Ahmad 3, Muhammad Bilal 4, Syed Muhammad Salman 5, Ian S. Butler 6, Tanveer A. Wani 7 and Seema Zargar 8
Round 1
Reviewer 1 Report
-In the Abstract part avoid the abbreviations like “E. coli, K. pneumonia and S. aureus”.
-In the Abstract part add in the method subsection, the name of the used anticlerical assay.
-Add briefly to the abstract part antibacterial results.
-Add statistical analysis subsection to the material and method section.
-Add statistical analysis to all obtained results of your study.
At the end of abstract part conclusions and future recommendations.
Nothing in the keyword mentioned about antibacterial activity.
- The whole manuscript needs language and editing corrections
- I recommend authors to compare their antibacterial results with antibiotics as positive control.
-throughout the whole manuscript and other Latin words must be in Italic manner.
The whole manuscript needs language and editing corrections by a native speaker
Author Response
Dear Reviewer
Thanks a lot for your valuable suggestions and corrections. we have tried our best to consider each and every point you have mentioned.
-In the Abstract part avoid the abbreviations like “E. coli, K. pneumonia and S. aureus”.
Author Response: Full names are now used instead of abbreviations: Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus
-In the Abstract part add in the method subsection, the name of the used anticlerical assay.
Author Response: Agar well diffusion method was used for antibacterial and using the sulforhodamine B (SRB) method was used for anticancer and are now added in the abstract as suggested.
-Add briefly to the abstract part antibacterial results.
Author Response: Done as suggested.
-Add statistical analysis subsection to the material and method section.
Author Response: Graph Pad Prism was applied for statistical analysis which is now mentioned in the material and method section.
-Add statistical analysis to all obtained results of your study.
Author Response: Added now as suggested.
At the end of abstract part conclusions and future recommendations.
Author Response: Recommendation added.
Nothing in the keyword mentioned about antibacterial activity.
Author Response: It is now added in the keywords.
- The whole manuscript needs language and editing corrections
Author Response: The whole manuscript is now thoroughly checked by an English Expert to omit the mistakes.
- I recommend authors to compare their antibacterial results with antibiotics as positive control.
Author Response: It can be seen in antibacterial Table 4, where Streptomycin is mentioned as positive reference drug.
-throughout the whole manuscript and other Latin words must be in Italic manner
Author Response: Done as suggested.
Reviewer 2 Report
This is a well-designed and performed study. They used several experimental approaches: chemical synthesis, physicochemical characterization, antibacterial activity assays, antitumor activity and in silico and molecular docking studies. The results obtained are very relevant.
Major point:
A conclusion must be added at the end both of the manuscript and abstract and the abstract.
Minor:
Line 296: “…of 2>3>4>1.” Should be “…of 2>3>1>4.”
Comments for author File: 
Comments.pdf
Author Response
Dear Reviewer, We are highly thankful for your valuable time and mentioned some critical points and suggestions. All the corrections made are yellow highlighted in the paper.
Comments. I suggest that authors provide a hypothesis. The justification for the use of dithiocarbamates for its incorporation to complexes with palladium is weak since use of references is missing.
Author Response: A paragraph including references (23-26) is added in the introduction part.
Please: Uniformize the use of words “cis-platin” or “cisplatin”.
Author Response: Ok suggestion incorporated. cisplatin is used.
Line 71. It is necessary to indicate the reference number of Khan et al.
Author Response: Ref. # 17-19 are now mentioned.
Line 113. Correct “dithiocarbamte” word.
Author Response: Corrected.
Line 304. Correct “positve” word.
Author Response: Corrected.
Line 182. Eliminate the abbreviation in the following phrase “The tested compounds having conc. 2 mg/mL”
Author Response: Done
Round 2
Reviewer 1 Report
The authors conducted all the required corrections and I have not any more corrections
