Implementation of an Enzyme Membrane Reactor to Intensify the α-O-Glycosylation of Resveratrol Using Cyclodextrins

The O-glycosylation of resveratrol increases both its solubility in water and its bioavailability while preventing its oxidation, allowing a more efficient use of this molecule as a bioactive ingredient in pharmaceutical and cosmetic applications. Resveratrol O-glycosides can be obtained by enzymatic reactions. Recent developments have made it possible to selectively obtain resveratrol α-glycosides from the β-cyclodextrin–resveratrol complex in water with a yield of 35%. However, this yield is limited by the partial hydrolysis of the resveratrol glycosides produced during the reaction. In this study, we propose to intensify this enzymatic reaction by coupling the enzymatic reactor to a membrane process. Firstly, membrane screening was carried out at the laboratory scale and led to the choice of a GE polymeric membrane with a cut-off of 1 kDa. This membrane allowed the retention of 65% of the β-cyclodextrin–resveratrol complex in the reaction medium and the transfer of 70% of the resveratrol α-O-glycosides in the permeate. In a second step, this membrane was used in an enzymatic membrane reactor and improved the yield of the enzymatic glycosylation up to 50%. View Full-Text

Keywords: process intensification; enzyme membrane reactor; enzymatic O-glycosylation; cyclodextrins; resveratrol

▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Ioannou, I.; Barboza, E.; Willig, G.; Marié, T.; Texeira, A.; Darme, P.; Renault, J.-H.; Allais, F. Implementation of an Enzyme Membrane Reactor to Intensify the α-O-Glycosylation of Resveratrol Using Cyclodextrins. Pharmaceuticals 2021, 14, 319. https://doi.org/10.3390/ph14040319

AMA Style

Ioannou I, Barboza E, Willig G, Marié T, Texeira A, Darme P, Renault J-H, Allais F. Implementation of an Enzyme Membrane Reactor to Intensify the α-O-Glycosylation of Resveratrol Using Cyclodextrins. Pharmaceuticals. 2021; 14(4):319. https://doi.org/10.3390/ph14040319

Chicago/Turabian Style

Ioannou, Irina, Eduardo Barboza, Gaëlle Willig, Thomas Marié, Andreïa Texeira, Pierre Darme, Jean-Hugues Renault, and Florent Allais. 2021. "Implementation of an Enzyme Membrane Reactor to Intensify the α-O-Glycosylation of Resveratrol Using Cyclodextrins" Pharmaceuticals 14, no. 4: 319. https://doi.org/10.3390/ph14040319

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Implementation of an Enzyme Membrane Reactor to Intensify the α-O-Glycosylation of Resveratrol Using Cyclodextrins

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Author Biographies

Further Information

Guidelines

MDPI Initiatives

Follow MDPI