Search Results (2,451)

Postharvest spoilage of fresh fruits demands efficient bio-based packaging films. Here, chitosan/gelatin (CG) films were incorporated with β-cyclodextrin-encapsulated clove essential oil microcapsules (β-CD@CEO MCs) at varying loadings. The results suggested that CEO encapsulation occurred within β-CD cavities and hydrogen-bond binding of MCs to the CG matrix. At 0.4% MCs, the composite film showed 60.11% higher tensile strength, excellent UV shielding, lower water vapor transmission rate, and strong antioxidant activity (DPPH 89.6%, ABTS 97.1), along with 58.21% biodegradation after 16 days of soil burial. In vitro release studies revealed a pH-responsive sustained release profile of CEO from the composite films, with faster release under acidic conditions (98.5% at pH 3.5 after 72 h) compared to neutral conditions (87.2% at pH 7.0), indicating the potential for targeted release on the weakly acidic grape surface. The film also exhibited significant antimicrobial effects against Botrytis cinerea, Penicillium gladioli, Staphylococcus aureus, and Escherichia coli. In table grape preservation, CG/MCs-0.4 film effectively delayed decay, reduced weight loss by 38.79%, maintained firmness and color, and preserved higher levels of soluble solids, titratable acidity, reducing sugars, and vitamin C compared to polyethylene packaging and untreated controls. Full article

A series of molecularly imprinted polymers (MIPs) based on hydroxypropyl-β-cyclodextrin (HPCD) crosslinked with 1,6-hexamethylene diisocyanate (HMD) or toluene diisocyanate (TDI), as well as hybrid chitosan–HPCD polymers crosslinked with genipin, were synthesized using levofloxacin and fluorescein as template molecules. The structure and spatial organization of the obtained materials were characterized by FTIR spectroscopy, FTIR microscopy mapping, and ζ-potential measurements. The influence of pH, crosslinker content, and template structure on sorption performance was investigated. All MIPs exhibited maximum sorption at pH 3.0. The highest sorption capacity toward levofloxacin was achieved for the LV–Chit–HPCD–Genipin_MIP (74.8 mg/g), whereas the fluorescein-imprinted FL–HPCD–TDI_MIP (1:1) demonstrated the highest sorption capacity (135.5 mg/g) and selectivity coefficient (84.1). Dynamic column experiments confirmed efficient analyte extraction, reducing the analyte concentration by more than 90% after ten loading cycles. All synthesized MIPs exhibited excellent regenerability, with less than 3% loss of sorption efficiency after ten consecutive sorption–desorption cycles. The applicability of the developed sorbents to real matrices was demonstrated using milk and blood plasma samples after minimal sample preparation. Fluorescein extraction efficiencies reached 97.6% and 93.6% for milk and plasma, respectively. The obtained results demonstrate that HPCD-based MIPs combine high sorption capacity, exceptional selectivity, operational stability, and applicability to complex biological matrices, making them promising materials for selective sample preparation, analyte preconcentration, and controlled drug delivery systems. Full article

Skin photoaging is closely associated with oxidative stress, inflammatory responses, and dysregulated collagen metabolism. Paeonol (Pae) possesses antioxidant and anti-inflammatory activities; however, its poor water solubility and short skin retention time limit its topical application. In this study, a transdermal delivery system based on a carboxymethyl chitosan (CMCS)/Carbomer 940 (Carb940) composite gel loaded with hydroxypropyl-β-cyclodextrin inclusion complexes of paeonol (Pae-CD) was developed. Pae-CD was prepared using an ultrasound-assisted saturated aqueous solution method, and the physicochemical properties, sustained-release behavior, transdermal permeation, antioxidant activity, and safety of Pae-CD/gel were evaluated. Furthermore, a mouse model of skin photoaging induced by combined ultraviolet A (UVA)/ultraviolet B (UVB) irradiation was established to investigate its reparative effects in vivo. The results showed that Pae-CD/gel exhibited a homogeneous three-dimensional porous structure, favorable sustained-release characteristics, enhanced skin retention capacity, and good cellular compatibility. In vivo experiments demonstrated that Pae-CD/gel markedly ameliorated ultraviolet-induced skin dryness, abnormal epidermal thickening, and dermal collagen loss. It also reduced oxidative stress and inflammatory factor levels, down-regulated matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-3 (MMP-3) expression, and promoted the restoration of collagen type I (COL-1) and hydroxyproline (HYP) levels. Systemic safety evaluation revealed no obvious toxicity. In summary, Pae-CD/gel exerts antioxidant and anti-inflammatory effects and regulates collagen metabolism by enhancing transdermal delivery and local retention, thereby providing a safe and effective topical delivery strategy for the repair of skin photoaging. Full article

Fruit- and vegetable-derived postbiotic powders represent promising functional ingredients; however, systematic evaluations of different drying technologies and drying aids for these products remain limited. In this study, Opuntia ficus-indica fruit juice was fermented using Lacticaseibacillus paracasei HNU502 and subsequently inactivated to prepare postbiotic broth, which was further processed into powder by vacuum freeze-drying and spray-drying with maltodextrin and β-cyclodextrin as drying aids. Results showed that the drying process and carrier formulation jointly influenced the structural, sensory, and antioxidant characteristics of cactus postbiotic powders. Vacuum freeze-drying combined with β-cyclodextrin exhibited favorable structural and sensory properties under the tested conditions, whereas spray-drying combined with β-cyclodextrin showed favorable antioxidant performance. Additionally, β-cyclodextrin-containing formulations showed favorable powder characteristics and functional properties under the tested conditions, although the effects were influenced by both carrier formulation and drying process. Among the tested formulations, vacuum freeze-drying combined with β-cyclodextrin exhibited the highest crystallinity (77.4%) and distinct structural features, as characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) analyses. The spray-dried β-cyclodextrin formulation achieved the highest sensory score (74.75), while exhibiting high radical scavenging activity. DSC analysis indicated that all formulations maintained thermal stability, with no exothermic degradation observed below 180 °C. These findings suggest that β-cyclodextrin-assisted drying strategies can influence the structural and functional characteristics of cactus postbiotic powders and that drying strategy selection should consider specific product quality requirements and processing feasibility. Full article

Quercetin is a nutraceutical flavonoid whose aqueous use is limited by poor solubility and chemical instability. Existing polysaccharide carriers may require chemical modification, crosslinkers, multicomponent formulations, or complex processing. Here, alkali-neutralization-induced β-1,3-1,6-glucan nanoparticles (r-glucan NPs), prepared from Aureobasidium pullulans K-1 glucan, were compared with β-cyclodextrin (β-CD), using native t-glucan as a composition-matched control. Spectroscopic analyses supported quercetin association with chiral, cavity-rich domains of r-glucan NPs. r-Glucan NPs showed higher quercetin loading than β-CD and increased apparent aqueous solubility/dispersibility to 1620 ± 15.3 µM, compared with 256 ± 0.52 µM for the native β-1,3-1,6-glucan control, 347 ± 16.4 µM for β-CD, and 7.11 ± 3.97 µM for free quercetin. The t-glucan control initially retained quercetin but showed limited aqueous dispersibility and formed a visible precipitate within 3 days in 1 vol% ethanol. Although carrier association reduced oxygen radical absorbance capacity (ORAC) activity on an equal-quercetin basis, r-glucan NPs yielded a maximum ORAC value of 11,600 ± 1200 µmol TE L⁻¹, compared with 815 ± 235 for β-CD and 368 ± 38.2 for free quercetin. r-Glucan NPs also improved apparent retention, photostability, stability at pH 6.8, and cellular antioxidant activity relative to free quercetin. These results support r-glucan NPs as food-compatible carriers for aqueous quercetin delivery. Full article

Low-molecular-weight lychee polyphenol (Lyp), a standardized oligomeric polyphenol derived from Litchi chinensis fruit, possesses potent antioxidant activity but is susceptible to physicochemical instability, which may limit its practical application. In this study, freeze-dried systems of Lyp with α-, β-, and γ-cyclodextrins (CDs) were prepared to investigate the effects of cyclodextrins on the physicochemical properties and antioxidant activity of Lyp. Powder X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, near-infrared spectroscopy, and solution-state NMR were employed to characterize the prepared systems. Freeze-drying produced amorphous Lyp/CD systems accompanied by enhanced thermal stability and changes in the hydrogen-bonding environment compared with the corresponding physical mixtures. Solution-state ¹H NMR and NOESY analyses suggested molecular association between Lyp constituents and cyclodextrins, with the βCD system exhibiting the most pronounced spectral changes. Consistent with these physicochemical findings, the freeze-dried βCD system showed the greatest enhancement of DPPH radical scavenging activity among the three cyclodextrins examined. These results suggest that βCD provides the most favorable molecular environment for Lyp, leading to improved physicochemical characteristics and antioxidant performance. The present findings demonstrate that freeze-drying combined with β-cyclodextrin is an effective strategy for improving the physicochemical performance and antioxidant functionality of low-molecular-weight lychee polyphenol. Full article

4-Chlorophenol (4-CP) is a persistent and highly toxic pollutant commonly found in groundwater. However, its efficient degradation remains challenging due to the rapid agglomeration of conventional zero-valent iron (ZVI) nanoparticles, their narrow pH operating range, and the environmental risks associated with synthetic chelating agents. To address these limitations, this study presents a rationally designed catalytic system integrating cyclodextrin polymer (CDP)-supported bimetallic Cu-Fe nanoparticles (Cu-Fe-CDP) with citric acid (CA) as a green complexing agent. The porous CDP matrix effectively mitigates nanoparticle agglomeration and provides abundant active sites, while the Fe-Cu bimetallic coupling accelerates electron transfer and iron corrosion. Critically, CA acts as a biocompatible ligand that sustains Fe(II)/Fe(III) redox cycling, expands the effective pH range, and enhances hydroxyl radical (·OH) generation. The system achieves 92.13% degradation of 4-CP within 80 min at pH 9.0 and nearly complete removal at pH values between 3.0 and 7.0. Mechanistic studies, including electron paramagnetic resonance (EPR) spectroscopy and radical quenching tests, confirm the dominance of ·OH radicals (82.67% inhibition by TBA) and the essential role of surface Fe(II)/Fe(III) cycling. The catalyst exhibits excellent reusability, broad-spectrum activity toward multiple pollutants, and sustained performance in real water matrices and long-term column tests with minimal metal leaching. This work demonstrates a chemically robust strategy for chlorophenol remediation using green citric acid and biodegradable CDP without exogenous oxidant addition, showing promise for further development toward practical applications. Full article

Active molecules derived from traditional Chinese medicine (TCM) represent a valuable source of lead compounds for modern drug discovery, demonstrating considerable potential in the prevention and treatment of chronic diseases, cancer therapy, and immune modulation. Nevertheless, their clinical translation is often impeded by intrinsic limitations such as poor aqueous solubility, low bioavailability, inadequate chemical stability, and significant gastrointestinal irritation. Cyclodextrins (CDs) and their derivatives, characterized by a hydrophobic internal cavity, are capable of forming inclusion complexes with TCM and their derived active constituents, thereby improving their physicochemical and pharmacokinetic profiles. Over the past five years, substantial progress has been made in this domain. CD-based inclusion strategies have been shown to markedly enhance the solubility and dissolution rate of poorly water-soluble TCM compounds, including flavonoids, alkaloids, and terpenoids. Moreover, this approach contributes to improved drug stability, effective taste masking, and the achievement of modified release profiles, such as sustained or targeted delivery, ultimately leading to enhanced therapeutic efficacy and reduced adverse effects. The development of novel CD derivatives and smart delivery systems has further broadened their application potential. This review summarizes recent advances in the CD-based encapsulation of TCM active molecules, highlighting key formulation strategies that address persistent challenges in the modernization of TCM. It also provides a foundation for future research and development in natural product-based therapeutics. Full article

Ketoprofen is a Biopharmaceutics Classification System (BCS) class II non-steroidal anti-inflammatory drug whose therapeutic performance is limited by its poor aqueous solubility, though complexation with cyclodextrins is a well-established strategy to overcome this limitation. The aim of this work was to prepare and characterize inclusion complexes of ketoprofen with HPβCD. Complexes were obtained by the solution (magnetic-stirring) method at ketoprofen–HPβCD molar ratios of 1:1 and 2:1 and were characterized by Fourier-transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) coupled with EDX, and computer-assisted dimensional analysis and statistics (AutoCAD/MathCAD). The bulk and tapped densities were used to derive the Carr index and the Hausner ratio, respectively. FT-IR showed attenuation and shifting of the characteristic C=O, C=C, and –CH bands of ketoprofen and the presence of the 1655 cm⁻¹ band in complexes demonstrates inclusion. DSC revealed a marked reduction of the ketoprofen melting endotherm, the degree of inclusion reaching 75% (1:1) and 62% (2:1). SEM and statistical analysis confirm, individualized particles, predominantly 20–100 µm in perimeter size. The dissolution test showed a faster release of the complexes than in the case of pure ketoprofen. Therefore, HPβCD forms stable inclusion complexes with ketoprofen having pharmacotechnical properties suitable for compressed tablets. Full article

Sustainable textile care formulations based on renewable and waste vegetable oils represent environmentally responsible alternatives to conventional laundry products while supporting circular bioeconomy strategies. However, the influence of formulation composition on EO transfer, textile persistence and multifunctional performance remains poorly understood. This study investigated the influence of formulation design on the physicochemical properties, encapsulation efficiency, EO transfer, textile persistence, washing performance and antibacterial activity of sustainable textile care formulations prepared from virgin and waste vegetable oils. All formulations exhibited appropriate physicochemical characteristics, including alkaline pH values (9.84–10.64), good foaming capacity and encapsulation efficiencies of 95.6–98.2%. Although encapsulation efficiency remained consistently high, formulation composition influenced EO transfer and persistence on textile substrates. Formulation V1-D exhibited the most balanced overall performance. Waste vegetable oil formulations achieved washing efficiencies of 86–92%, comparable to those of virgin oil formulations, while maintaining EO persistence on textile substrates. The developed soap formulations also exhibited pronounced antibacterial activity against Staphylococcus aureus and Escherichia coli. These findings indicate that rational formulation design, rather than encapsulation efficiency alone, primarily determines the overall performance of sustainable multifunctional textile care formulations by balancing washing efficiency, EO transfer, textile persistence, and the antibacterial activity of the developed soap formulations. Full article

Background/Objectives: Chronic complications of diabetes mellitus are closely associated with increased oxidative stress and the formation of advanced glycation end products (AGEs). This study aimed to investigate the antioxidant and antiglycation potential of hesperetin-7-O-glucoside (HCD) and diosmetin-7-O-glucoside (DCD) formulations complexed with cyclodextrins, using in vitro and in silico experimental models to evaluate their efficacy in mitigating hyperglycemia-induced molecular damage. Methods: Antioxidant activity was assessed using chemical and erythrocyte-based oxidative stress models, whereas antiglycation activity was evaluated in BSA–fructose, BSA–methylglyoxal, and arginine–methylglyoxal models. Results: Both formulations showed measurable antioxidant effects, with concentration-dependent behavior observed in some of the evaluated assays. In the DPPH assay, HCD and DCD achieved maximum inhibition values of 30.71% and 26.61%, respectively. Furthermore, DCD exhibited higher total antioxidant capacity (102.80 µg vitamin C equivalents/mL) and nitric oxide scavenging activity (37.89%) than HCD. In a cellular model, both formulations (200 µg/mL) significantly reduced AAPH-induced hemolysis, with DCD providing superior protection (7.05% vs. 16.63% for HCD). Under oxidative stress induced by high glucose concentration in erythrocytes, HCD and DCD reduced non-protein thiol levels, and HCD significantly increased catalase enzyme activity. Regarding antiglycation activity, DCD demonstrated superior efficacy relative to HCD in the BSA-fructose system, achieving 43.23% inhibition. DCD also displayed concentration-dependent inhibition of fructosamine formation (up to 47.99%) and BSA glycation by methylglyoxal (up to 45.20%). Both formulations significantly reduced carbonylated protein levels and preserved free thiol groups. In the arginine–methylglyoxal model, DCD and HCD reached 44.03% and 48.82% inhibition, respectively. Molecular docking revealed high binding affinity of both flavonoids to the protein active site (−9.00 kcal/mol for hesperetin-7-O-glucoside and −9.04 kcal/mol for diosmetin-7-O-glucoside), suggesting a structural protective role. Conclusions: The HCD and DCD formulations demonstrated antioxidant and antiglycation activities that may contribute to attenuating molecular alterations associated with chronic hyperglycemia through complementary mechanisms, including antioxidant effects, protection against protein carbonylation, and inhibition of glycation. While these findings highlight the potential of the evaluated formulations, additional mechanistic and in vivo studies are required to establish their pharmacological applicability. Full article

Nano- and pico-bubble water (NPB), containing hydrogen or ozone, is widely used as a cleaning agent due to its bactericidal and antiviral properties. However, some products—such as certain hydrogen waters—contain only large bubbles or have an extremely low bubble count, making them sometimes indistinguishable from ordinary drinking water. To accurately evaluate NPB activity, we developed a method for producing ultra-nano–pico-bubble water (NanoGAS water [NGW]), an ultra-fine bubble water that is stable and non-volatile over extended periods. By combining a mixed gas–liquid fluid rotary mixer and a shear filter, we produced ultra-fine bubbles that could be sealed in water. This method produced bubbles that remained stable in water even after 10 years since production. NGW has been clinically evaluated as a solvent for fecal microbiota transplantation (FMT) and has been demonstrated to be effective at improving bacterial engraftment in the intestinal tract in patients with autism spectrum disorder (ASD). Furthermore, encapsulating specific gases (hydrogen and ozone) can achieve more diverse effects. In this study, we evaluated the aroma-encapsulating effects, as well as the strength and persistence of the antimicrobial activity, of novel NGW formulations (Air-NGW, H₂-NGW, S-O₃-NGW, and L-O₃-NGW). Both H₂-NGW and O₃-NGW generated in this study demonstrated slight inclusion activity with volatile aromatic compounds (citral). Furthermore, both H₂-NGW (at ≥10% dilution) and O₃-NGW (even at a 1% dilution) exhibited sustained antibacterial efficacy against general viable bacteria for 24 weeks. Moreover, additive effects were observed when combined with antibacterial and antiviral compounds (polyoxometalates) developed by the authors. While further consideration, including cost-effectiveness, is needed to translate these findings into practical applications, they provide a fundamental framework for future research. Full article

Cyclodextrin (CD)-based supramolecular nanofibers (NFs) have emerged as an advanced class of multifunctional materials for active food packaging by integrating host–guest supramolecular chemistry with electrospun nanofibrous architectures. The unique hydrophobic cavity and hydrophilic exterior of CDs enable the encapsulation of a wide range of bioactive compounds, including essential oils, natural antioxidants, antimicrobials, and volatile active agents, thereby enhancing their solubility, stability, controlled release, and preservation efficacy. This review comprehensively discusses the molecular structure and inclusion complexation mechanisms of CDs, recent advances in polymer-assisted and polymer-free electrospinning strategies, and the design of CD-based supramolecular nanofibers for food preservation. Particular emphasis is placed on the relationship between fiber morphology, supramolecular interactions, and controlled release behavior, which collectively govern antimicrobial, antioxidant, moisture management, and barrier properties. Recent developments involving biodegradable polymers, hybrid nanofibrous systems, and cyclodextrin-based metal–organic frameworks (CD-MOFs) are critically summarized, highlighting their roles in improving encapsulation efficiency, mechanical stability, and multifunctional performance. The review further compares CD-based nanofibers with other advanced encapsulation technologies, including liposomes, solid lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, polymeric nanoparticles, microspheres, and conventional MOFs, providing a comprehensive evaluation of their loading capacity, release kinetics, scalability, cost, and regulatory suitability for food-contact applications. Representative applications in the preservation of fruits, vegetables, meat, seafood, dairy products, and bakery products demonstrate significant improvements in microbial inhibition, oxidation resistance, ethylene and volatile organic compound adsorption, and shelf-life extension through sustained delivery of natural preservatives. Ultimately, the current challenges, including large-scale manufacturing, long-term stability, regulatory approval, and commercialization, are discussed together with future directions, focusing on smart packaging, stimuli-responsive delivery systems, intelligent sensing, biodegradable multifunctional materials, and sustainable industrial implementation. Full article

Background/Objectives: The extraction and encapsulation of Tanacetum parthenium L. (feverfew) represent highly relevant approaches for maximizing the recovery and protection of its bioactive phytocomplexes, particularly the main active compound, parthenolide (PAR). The present study aimed to develop and optimize a cyclodextrin-based extraction and encapsulation method for T. parthenium to improve the chemical and physicochemical properties and biological activity of bioactive-rich phytocomplexes. Methods: The extraction process was optimized by comparing conventional maceration (ME) and ultrasound-assisted extraction (UE), using different ethanol–water mixtures as extractants, with and without hydroxypropyl-β-cyclodextrin (CD) as an extraction enhancer. The highest PAR yield (approximately 6.3 mg/g dried biomass), combined with enhanced total polyphenol and flavonoid contents, was achieved using 50% (v/v) ethanol with 0.5% CD under UE. The optimized extract was spray-dried to produce powder formulations, with or without carrier material, which have been characterized for their technological, chemical, physicochemical, structural, and biological properties. Results: The inclusion of CD significantly improved process yield (approximately 65% compared to 47% for the non-encapsulated extract) and reduced residual moisture content, indicating enhanced drying efficiency and improved powder stability. Furthermore, the encapsulated formulation demonstrated preserved antioxidant and anti-inflammatory activity in cell-based assays. Conclusions: The developed CD-based encapsulation method represents a promising strategy for stabilizing feverfew bioactives and enhancing their potential applicability in pharmaceutical and dermo-cosmetic formulations. Full article