ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae
Abstract
1. Introduction
2. Materials and Methods
2.1. LAMP Primer Design and Conservation Analysis
2.2. LAMP Reaction Conditions
2.3. Analytical Performance of Low-Buffered LAMP
2.4. Gel Electrophoresis
2.5. Fabrication and Working Principle of ISFET Sensors
2.6. ISFET-Based H+ Sensing System
2.7. Detection of Clinical Samples
3. Results
3.1. Performance of ISFET-Based Sensing Platform
3.2. LAMP Primer Design and Conservation Analysis
3.3. Optimization and Performance of LAMP Assay
3.4. MP Detection with ISFET Sensors with pH-LAMP
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zou, J.; Hu, J.; Shen, Y.; Zhang, L.; Bai, W.; Wang, L.; Li, J.; Yan, L.; Zhang, Z.; Bai, H.; et al. ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae. Sensors 2025, 25, 1562. https://doi.org/10.3390/s25051562
Zou J, Hu J, Shen Y, Zhang L, Bai W, Wang L, Li J, Yan L, Zhang Z, Bai H, et al. ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae. Sensors. 2025; 25(5):1562. https://doi.org/10.3390/s25051562
Chicago/Turabian StyleZou, Jie, Jie Hu, Yan Shen, Limei Zhang, Weiyi Bai, Lei Wang, Jianlong Li, Lin Yan, Zhifeng Zhang, Hao Bai, and et al. 2025. "ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae" Sensors 25, no. 5: 1562. https://doi.org/10.3390/s25051562
APA StyleZou, J., Hu, J., Shen, Y., Zhang, L., Bai, W., Wang, L., Li, J., Yan, L., Zhang, Z., Bai, H., & Hu, W. (2025). ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae. Sensors, 25(5), 1562. https://doi.org/10.3390/s25051562