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Communication

Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer

1
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan
2
Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University (TWIns), 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Antonios Kelarakis
Sensors 2021, 21(9), 3115; https://doi.org/10.3390/s21093115
Received: 19 April 2021 / Revised: 27 April 2021 / Accepted: 27 April 2021 / Published: 30 April 2021
(This article belongs to the Section Chemical Sensors)
This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 104 colony-forming units (CFU)·mL−1. Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria’s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria. View Full-Text
Keywords: bacteria detection; E. coli; S. aureus; dendrimer; phenylboronic acid; fluorescence measurement bacteria detection; E. coli; S. aureus; dendrimer; phenylboronic acid; fluorescence measurement
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MDPI and ACS Style

Mikagi, A.; Tsurufusa, R.; Tsuchido, Y.; Hashimoto, T.; Hayashita, T. Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer. Sensors 2021, 21, 3115. https://doi.org/10.3390/s21093115

AMA Style

Mikagi A, Tsurufusa R, Tsuchido Y, Hashimoto T, Hayashita T. Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer. Sensors. 2021; 21(9):3115. https://doi.org/10.3390/s21093115

Chicago/Turabian Style

Mikagi, Ayame, Riho Tsurufusa, Yuji Tsuchido, Takeshi Hashimoto, and Takashi Hayashita. 2021. "Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer" Sensors 21, no. 9: 3115. https://doi.org/10.3390/s21093115

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