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Sensors 2019, 19(2), 422; https://doi.org/10.3390/s19020422

Low-Temperature Storage Improves the Over-Time Stability of Implantable Glucose and Lactate Biosensors

Medical, Surgical and Experimental Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, Italy
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Received: 21 December 2018 / Revised: 9 January 2019 / Accepted: 18 January 2019 / Published: 21 January 2019
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Abstract

Molecular biomarkers are very important in biology, biotechnology and even in medicine, but it is quite hard to convert biology-related signals into measurable data. For this purpose, amperometric biosensors have proven to be particularly suitable because of their specificity and sensitivity. The operation and shelf stability of the biosensor are quite important features, and storage procedures therefore play an important role in preserving the performance of the biosensors. In the present study two different designs for both glucose and lactate biosensor, differing only in regards to the containment net, represented by polyurethane or glutharaldehyde, were studied under different storage conditions (+4, −20 and −80 °C) and monitored over a period of 120 days, in order to evaluate the variations of kinetic parameters, as VMAX and KM, and LRS as the analytical parameter. Surprisingly, the storage at −80 °C yielded the best results because of an unexpected and, most of all, long-lasting increase of VMAX and LRS, denoting an interesting improvement in enzyme performances and stability over time. The present study aimed to also evaluate the impact of a short-period storage in dry ice on biosensor performances, in order to simulate a hypothetical preparation-conservation-shipment condition. View Full-Text
Keywords: glucose; lactate; biosensors; low-temperature storage; over-time stability glucose; lactate; biosensors; low-temperature storage; over-time stability
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Puggioni, G.; Calia, G.; Arrigo, P.; Bacciu, A.; Bazzu, G.; Migheli, R.; Fancello, S.; Serra, P.A.; Rocchitta, G. Low-Temperature Storage Improves the Over-Time Stability of Implantable Glucose and Lactate Biosensors. Sensors 2019, 19, 422.

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