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Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc
Open AccessArticle

Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform

College of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-746, Korea
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Author to whom correspondence should be addressed.
Sensors 2019, 19(14), 3207; https://doi.org/10.3390/s19143207
Received: 20 May 2019 / Revised: 5 July 2019 / Accepted: 17 July 2019 / Published: 20 July 2019
(This article belongs to the Special Issue Sensors and Lab-on-a-Chip)
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Abstract

Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurement and heating system in non-contact with the surface of LOD, which is a prerequisite to successful realization of DNA amplification especially with a rotatable disc. This study presents a Lab-on-a-disc (LOD)-based automatic loop-mediated isothermal amplification (LAMP) system, where a thermochromic coating (<~420 µm) was used to distantly measure the chamber’s temperature and a micro graphite film was integrated into the chamber to remotely absorb laser beam with super high efficiency. We used a deep learning network to more consistently analyze the product of LAMP than we could with the naked eye. Consequently, both temperature heating and measurement were carried out without a physical contact with the surface of LOD. The experimental results show that the proposed approach, which no previous work has attempted, was highly effective in realizing LAMP in LOD. View Full-Text
Keywords: lab-on-a-disc; loop-mediated isothermal amplification; point-of-care testing; hydroxy naphthol blue; convolutional neural network lab-on-a-disc; loop-mediated isothermal amplification; point-of-care testing; hydroxy naphthol blue; convolutional neural network
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lim, D.Y.S.; Seo, M.-J.; Yoo, J.C. Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform. Sensors 2019, 19, 3207.

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