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Sensors 2018, 18(7), 2168; https://doi.org/10.3390/s18072168

Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors

1
Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (SA), Italy
2
Institute of Crystallography of the National Council of Research (CNR), 00015 Monterotondo Scalo, Italy
*
Author to whom correspondence should be addressed.
Received: 12 June 2018 / Revised: 2 July 2018 / Accepted: 4 July 2018 / Published: 5 July 2018
(This article belongs to the Special Issue Smart Biosensing at BBMEC 12, Rome)
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Abstract

In this work, the development of an impedimetric label-free immunosensor for the detection of Escherichia coli O157:H7 is reported. Different immobilization techniques of monoclonal anti-E. coli were tested, in order to reach the very low limit of detections. The comparison between the immobilization procedures underlined the advantages of the oriented procedure and the use of a dendrimer, which allowed for immobilizing a higher number of antibody units, reaching a very high sensitivity. However, the use of activated ferrocene as electron-transferring mediator, which improved the electrical properties of the system, resulted in a very low limit of detection equal to 3 cfu/mL. This immunosensor was used to analyze milk and meat samples obtaining a good agreement with the results of the ELISA methods. View Full-Text
Keywords: label-free immunosensor; Escherichia coli 0157:H7; electrochemical impedance spectroscopy label-free immunosensor; Escherichia coli 0157:H7; electrochemical impedance spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Malvano, F.; Pilloton, R.; Albanese, D. Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors. Sensors 2018, 18, 2168.

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