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Sensors 2016, 16(9), 1489;

A Microchip for Integrated Single-Cell Gene Expression Profiling and Genotoxicity Detection

School of Mechanical Engineering and Automation, Fuzhou University, Fujian 350116, China
Author to whom correspondence should be addressed.
Academic Editors: Fan-Gang Tseng and Tuhin Subhra Santra
Received: 16 July 2016 / Revised: 28 August 2016 / Accepted: 6 September 2016 / Published: 14 September 2016
(This article belongs to the Special Issue Biomicrofluidics)
Full-Text   |   PDF [2961 KB, uploaded 14 September 2016]   |  


Microfluidics-based single-cell study is an emerging approach in personalized treatment or precision medicine studies. Single-cell gene expression holds a potential to provide treatment selections with maximized efficacy to help cancer patients based on a genetic understanding of their disease. This work presents a multi-layer microchip for single-cell multiplexed gene expression profiling and genotoxicity detection. Treated by three drug reagents (i.e., methyl methanesulfonate, docetaxel and colchicine) with varied concentrations and time lengths, individual human cancer cells (MDA-MB-231) are lysed on-chip, and the released mRNA templates are captured and reversely transcribed into single strand DNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), cyclin-dependent kinase inhibitor 1A (CDKN1A), and aurora kinase A (AURKA) genes from single cells are amplified and real-time quantified through multiplex polymerase chain reaction. The microchip is capable of integrating all steps of single-cell multiplexed gene expression profiling, and providing precision detection of drug induced genotoxic stress. Throughput has been set to be 18, and can be further increased following the same approach. Numerical simulation of on-chip single cell trapping and heat transfer has been employed to evaluate the chip design and operation. View Full-Text
Keywords: single-cell analysis; integrated multiplex RT-qPCR; microfluidics single-cell analysis; integrated multiplex RT-qPCR; microfluidics

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Dong, H.; Sun, H. A Microchip for Integrated Single-Cell Gene Expression Profiling and Genotoxicity Detection. Sensors 2016, 16, 1489.

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