A R2R3-MYB Transcription Factor of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis
Abstract
1. Introduction
2. Results
2.1. The Expression of GmMYB62 Continues to Increase in Soybean Seed-Coat from Yellow to Black
2.2. Expression Pattern and Phylogenetic Analysis of GmMYB62
2.3. Generation and Confirmation of GmMYB62-Overexpressing Transgenic Arabidopsis Plants
2.4. Overexpression of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis
2.5. GmMYB62 Affects the Expression of Genes Related to Anthocyanin Biosynthesis and Seed Size Regulation in Arabidopsis
2.6. GmMYB62 Affects the Expression of Genes Involved in Cell Cycle and Seed Size Regulation in Arabidopsis
3. Discussion
3.1. GmMYB62 Is a R2R3-MYB Transcription Factor That Govern Anthocyanin Accumulation in Plants
3.2. The Transcription Factor of GmMYB62 Specifically Regulates the Expression of Genes in the Pathway of Anthocyanin Synthesis
3.3. R2R3-MYB Transcription Factor of GmMYB62 Was Involved in Seed Size Regulation
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Bioinformatics Analysis of GmMYB62
4.3. Phylogenetic and Domain Analysis of MYB62 in Multispecies
4.4. DNA, RNA Extraction and Quantitative Reverse Transcription PCR (qRT-PCR)
4.5. Subcellular Localization
4.6. Generation and Selection of GmMYB62-Overexpressing Transgenic Arabidopsis Plants
4.7. Seed Size and Weight Measurement
4.8. Relative Anthocyanin Content Measurement
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, B.-Y.; Yuan, J.-B.; Gu, J.-B.; Li, C.; Lin, Y.; Zhang, Y.-H.; Zhang, B.-H.; Wang, Y.-H.; Ye, X.; Li, Y.; et al. A R2R3-MYB Transcription Factor of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis. Int. J. Mol. Sci. 2025, 26, 3457. https://doi.org/10.3390/ijms26083457
Zhao B-Y, Yuan J-B, Gu J-B, Li C, Lin Y, Zhang Y-H, Zhang B-H, Wang Y-H, Ye X, Li Y, et al. A R2R3-MYB Transcription Factor of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis. International Journal of Molecular Sciences. 2025; 26(8):3457. https://doi.org/10.3390/ijms26083457
Chicago/Turabian StyleZhao, Bi-Yao, Jian-Bo Yuan, Jin-Bao Gu, Cong Li, Yan Lin, Yu-Hang Zhang, Bai-Hong Zhang, Yin-Hua Wang, Xing Ye, Yang Li, and et al. 2025. "A R2R3-MYB Transcription Factor of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis" International Journal of Molecular Sciences 26, no. 8: 3457. https://doi.org/10.3390/ijms26083457
APA StyleZhao, B.-Y., Yuan, J.-B., Gu, J.-B., Li, C., Lin, Y., Zhang, Y.-H., Zhang, B.-H., Wang, Y.-H., Ye, X., Li, Y., Wang, Z.-Y., & Zhong, T.-X. (2025). A R2R3-MYB Transcription Factor of GmMYB62 Regulates Seed-Coat Color and Seed Size in Arabidopsis. International Journal of Molecular Sciences, 26(8), 3457. https://doi.org/10.3390/ijms26083457