Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals
4.3. Cell Culture
4.4. Human Atopic Dermatitis (AD) Skin
4.5. Development of DFE-Induced Atopic Skin Inflammation in Mouse Ear
4.6. Immunoprecipitation and Immunoblotting
4.7. Histological Analysis
4.8. Immunohistochemistry Analysis
4.9. qPCR
4.10. ELISA
4.11. Transfection of Keratinocytes with Small Interfering RNA (siRNA)
4.12. Transfection
4.13. Histamine Assay
4.14. Flow Cytometry Analysis
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jin, M.; Bang, J.S.; Ha, D.-L.; Kim, J.Y.; Park, K.D.; Lee, W.J.; Lee, S.-J.; Choi, J.K.; Choi, Y.-A.; Jang, Y.H.; et al. Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1. Int. J. Mol. Sci. 2023, 24, 5228. https://doi.org/10.3390/ijms24065228
Jin M, Bang JS, Ha D-L, Kim JY, Park KD, Lee WJ, Lee S-J, Choi JK, Choi Y-A, Jang YH, et al. Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1. International Journal of Molecular Sciences. 2023; 24(6):5228. https://doi.org/10.3390/ijms24065228
Chicago/Turabian StyleJin, Meiling, Jin Seon Bang, Dae-Lyong Ha, Jun Young Kim, Kyung Duck Park, Weon Ju Lee, Seok-Jong Lee, Jin Kyeong Choi, Young-Ae Choi, Yong Hyun Jang, and et al. 2023. "Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1" International Journal of Molecular Sciences 24, no. 6: 5228. https://doi.org/10.3390/ijms24065228
APA StyleJin, M., Bang, J. S., Ha, D.-L., Kim, J. Y., Park, K. D., Lee, W. J., Lee, S.-J., Choi, J. K., Choi, Y.-A., Jang, Y. H., & Kim, S.-H. (2023). Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1. International Journal of Molecular Sciences, 24(6), 5228. https://doi.org/10.3390/ijms24065228