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Article

A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method

by
Jérôme Guitton
1,2,3,
Floriane Gavotto
1,4,
Emeline Cros-Perrial
2,
Lars Petter Jordheim
2,5 and
Christelle Machon
1,2,5,*
1
Biochemistry and Toxicology Laboratory, Lyon Sud Hospital, University Hospital of Lyon, CEDEX, 69495 Pierre-Bénite, France
2
Inserm U1052, CNRS UMR5286 Centre de Recherche en Cancérologie de Lyon, 69000 Lyon, France
3
Toxicology Laboratory, Faculty of Pharmacy, University of Lyon, 69008 Lyon, France
4
Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4367 Belvaux, Luxembourg
5
Analytical Chemistry Laboratory, Faculty of Pharmacy, University of Lyon, 69008 Lyon, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(4), 3770; https://doi.org/10.3390/ijms24043770
Submission received: 22 December 2022 / Revised: 31 January 2023 / Accepted: 8 February 2023 / Published: 13 February 2023
(This article belongs to the Special Issue Liquid Chromatography-Mass Spectrometry in Metabolomics)
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Abstract

Performances of metabolomic methods have been widely studied on biological matrices such as serum, plasma, and urine; but much less on in vitro cell extracts. While the impact of cell culture and sample preparation on results are well-described, the specific effect of the in vitro cellular matrix on the analytical performance remains uncertain. The aim of the present work was to study the impact of this matrix on the analytical performance of an LC-HRMS metabolomic method. For this purpose, experiments were performed on total extracts from two cell lines (MDA-MB-231 and HepaRG) using different cell numbers. Matrix effects, carryover, linearity, and variability of the method were studied. Results showed that the performances of the method depend on the nature of the endogenous metabolite, the cell number, and the nature of the cell line. These three parameters should, therefore, be considered for the processing of experiments and the interpretation of results depending on whether the study focuses on a limited number of metabolites or aims to establish a metabolic signature.
Keywords: targeted metabolomic; liquid chromatography; high resolution mass spectrometry; cell extracts; analytical performances; matrix effect targeted metabolomic; liquid chromatography; high resolution mass spectrometry; cell extracts; analytical performances; matrix effect

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MDPI and ACS Style

Guitton, J.; Gavotto, F.; Cros-Perrial, E.; Jordheim, L.P.; Machon, C. A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method. Int. J. Mol. Sci. 2023, 24, 3770. https://doi.org/10.3390/ijms24043770

AMA Style

Guitton J, Gavotto F, Cros-Perrial E, Jordheim LP, Machon C. A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method. International Journal of Molecular Sciences. 2023; 24(4):3770. https://doi.org/10.3390/ijms24043770

Chicago/Turabian Style

Guitton, Jérôme, Floriane Gavotto, Emeline Cros-Perrial, Lars Petter Jordheim, and Christelle Machon. 2023. "A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method" International Journal of Molecular Sciences 24, no. 4: 3770. https://doi.org/10.3390/ijms24043770

APA Style

Guitton, J., Gavotto, F., Cros-Perrial, E., Jordheim, L. P., & Machon, C. (2023). A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method. International Journal of Molecular Sciences, 24(4), 3770. https://doi.org/10.3390/ijms24043770

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